The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by <em>Chryseobacterium daeguense </em>W6

Weijie Liu; Liu Cong; Hongli Yuan; Jinshui Yang; Weijie Liu; Liu Cong; Hongli Yuan; Jinshui Yang

doi:10.3934/environsci.2015.2.169

AIMS Environmental Science

2015, Volume 2, Issue 2: 169-179. doi: 10.3934/environsci.2015.2.169

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Research article

The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by Chryseobacterium daeguense W6

1.
School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China;
2.
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China

Received: 23 January 2015 Accepted: 10 March 2015 Published: 11 March 2015

In recent years, several novel cation-independent bioflocculants have been reported, which can avoid the secondary contamination caused by addition of cations. However, compared with cation-dependent bioflocculants, the flocculating mechanism of cation-independent bioflocculants is largely unknown. In this study, a cation-independent bioflocculant MBF-W6 produced by Chryseobacterium daeguense W6 was used as a model to investigate the flocculating mechanism. The results showed that the major flocculating component of MBF-W6 is a complex of proteins and polysaccharides. The zeta potential results indicated that kaolin clay particles were not precipitated due to charge neutralization and the bridging mediated by cations did not play a major role in the flocculating process. These results are consistent with the fact that MBF-W6 is a cation-independent bioflocculant. Further scanning electron microscopic observation showed that MBF-W6 induced flocs formed tight packed structure, suggesting that the kaolin clay particles maybe directly attached and bridged by bioflocculant MBF-W6. In addition, we also found out that Fe³⁺ ions inhibit the flocculating activity of MBF-W6 by affecting –COO^- and –NH groups. Therefore this study can improve our understanding on flocculating mechanism of cation-independent bioflocculants.
- bioflocculant,
- cation-independent,
- flocculating mechanism,
- polysaccharide,
- Chryseobacterium daeguense
Citation: Weijie Liu, Liu Cong, Hongli Yuan, Jinshui Yang. The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by Chryseobacterium daeguense W6[J]. AIMS Environmental Science, 2015, 2(2): 169-179. doi: 10.3934/environsci.2015.2.169

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Abstract

In recent years, several novel cation-independent bioflocculants have been reported, which can avoid the secondary contamination caused by addition of cations. However, compared with cation-dependent bioflocculants, the flocculating mechanism of cation-independent bioflocculants is largely unknown. In this study, a cation-independent bioflocculant MBF-W6 produced by Chryseobacterium daeguense W6 was used as a model to investigate the flocculating mechanism. The results showed that the major flocculating component of MBF-W6 is a complex of proteins and polysaccharides. The zeta potential results indicated that kaolin clay particles were not precipitated due to charge neutralization and the bridging mediated by cations did not play a major role in the flocculating process. These results are consistent with the fact that MBF-W6 is a cation-independent bioflocculant. Further scanning electron microscopic observation showed that MBF-W6 induced flocs formed tight packed structure, suggesting that the kaolin clay particles maybe directly attached and bridged by bioflocculant MBF-W6. In addition, we also found out that Fe³⁺ ions inhibit the flocculating activity of MBF-W6 by affecting –COO^- and –NH groups. Therefore this study can improve our understanding on flocculating mechanism of cation-independent bioflocculants.

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