An experimental method of bioimpedance measurement and analysis for discriminating tissues of fruit or vegetable

Arijit Roy; Abhishek Mallick; Surajit Das; Abhijit Aich; Arijit Roy; Abhishek Mallick; Surajit Das; Abhijit Aich

doi:10.3934/biophy.2020004

AIMS Biophysics

2020, Volume 7, Issue 1: 41-53. doi: 10.3934/biophy.2020004

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An experimental method of bioimpedance measurement and analysis for discriminating tissues of fruit or vegetable

Department of Electronics, West Bengal State University, Barasat, Kolkata, India 700126

Received: 22 February 2020 Accepted: 22 March 2020 Published: 24 March 2020

It is difficult to apply bioimpedance technique to discriminate plant tissues due to the large variability of Cole parameters. A novel electronic method for characterizing tissue of fruit or vegetable with discrimination capability is presented. A specially designed electrode-pair is used to measure the bioimpedance and Cole parameters are extracted. The relaxation time which is a fundamental property of a given tissue is then estimated and analysis of variance is performed to gain statistical relevance. Bioimpedance is measured for apple and potato tissues in the frequency range of 1 Hz to 1 MHz and rigorous data analyses are performed. With 99% confidence, it is shown than the relaxation time is independent of position of measurement and is specific for a given fruit or vegetable. Further, the tissues of different species of a given fruit (or vegetable) is shown to be discriminated statistically at the confidence level of 98%. An excellent correlation is shown between the bioimpedance parameters and the parameters of cellular structure. Experimental data shows that our technique can be generalized to distinguish unhealthy or treated fruits (or vegetables) and therefore bearing immense applicability in the characterization of plant tissue.
- bioimpedance,
- Cole model,
- discrimination,
- electrical,
- measurement,
- tissue characterization
Citation: Arijit Roy, Abhishek Mallick, Surajit Das, Abhijit Aich. An experimental method of bioimpedance measurement and analysis for discriminating tissues of fruit or vegetable[J]. AIMS Biophysics, 2020, 7(1): 41-53. doi: 10.3934/biophy.2020004

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Abstract

It is difficult to apply bioimpedance technique to discriminate plant tissues due to the large variability of Cole parameters. A novel electronic method for characterizing tissue of fruit or vegetable with discrimination capability is presented. A specially designed electrode-pair is used to measure the bioimpedance and Cole parameters are extracted. The relaxation time which is a fundamental property of a given tissue is then estimated and analysis of variance is performed to gain statistical relevance. Bioimpedance is measured for apple and potato tissues in the frequency range of 1 Hz to 1 MHz and rigorous data analyses are performed. With 99% confidence, it is shown than the relaxation time is independent of position of measurement and is specific for a given fruit or vegetable. Further, the tissues of different species of a given fruit (or vegetable) is shown to be discriminated statistically at the confidence level of 98%. An excellent correlation is shown between the bioimpedance parameters and the parameters of cellular structure. Experimental data shows that our technique can be generalized to distinguish unhealthy or treated fruits (or vegetables) and therefore bearing immense applicability in the characterization of plant tissue.

Acknowledgments

The authors would like to thank to Dr. Debaprasad Mandal (Department of Zoology, West Bengal State University) for valuable discussion on samples used in this research work. The authors would also like to thank to Dr. A.R. Sherpa (Department of Botany, West Bengal State University) for providing microscope facility. The second author would like to acknowledge DST, Govt. of India for providing fellowship under “DST Inspire Fellow” scheme.

Conflict of interest

The authors declare no conflicts of interest.

References

[1]	Greenham CG, Norris DO, Brock RD, et al. (1952) Some electrical differences between healthy and virus-infected potato tubers. Nature 169: 973-974. doi: 10.1038/169973a0
[2]	Greenham CG, Helms K, Müller WJ (1978) Influence of virus inflections on impedance parameters. J Exp Bot 29: 867-877. doi: 10.1093/jxb/29.4.867
[3]	Greenham CG, Daday H (1957) Electrical determination of cold hardiness in Trifolium repens L. and Medicago sativa L. Nature 180: 541-543. doi: 10.1038/180541a0
[4]	Jackson PJ, Harker FR (2000) Apple bruise detection by electrical impedance measurement. HortScience 35: 104-107. doi: 10.21273/HORTSCI.35.1.104
[5]	Wu L, Ogawa Y, Tagawa A (2008) Electrical impedance spectroscopy analysis of eggplant pulp and effects of drying and freezing–thawing treatments on its impedance characteristics. J Food Eng 87: 274-280. doi: 10.1016/j.jfoodeng.2007.12.003
[6]	Jha SN, Narsaiah K, Basediya AL, et al. (2011) Measurement techniques and application of electrical properties for nondestructive quality evaluation of foods—a review. J Food Sci Tech 48: 387-411. doi: 10.1007/s13197-011-0263-x
[7]	Khaled DE, Novas N, Gazquez JA, et al. (2015) Fruit and vegetable quality assessment via dielectric sensing. Sensors 15: 15363-15397. doi: 10.3390/s150715363
[8]	Chowdhury A, Singh P, Bera TK, et al. (2017) Electrical impedance spectroscopic study of mandarin orange during ripening. J Food Meas Charact 11: 1654-1664. doi: 10.1007/s11694-017-9545-y
[9]	Zhang MIN, Willison JHM (1993) Electrical impedance analysis in plant tissues8. J Exp Bot 44: 1369-1375. doi: 10.1093/jxb/44.8.1369
[10]	Grimnes S, Martinsen OG (2004) Cole electrical impedance model-a critique and an alternative. IEEE T Biomed Eng 52: 132-135. doi: 10.1109/TBME.2004.836499
[11]	Elwakil AS, Maundy B (2010) Extracting the Cole-Cole impedance model parameters without direct impedance measurement. Electron Lett 46: 1367-1368. doi: 10.1049/el.2010.1924
[12]	Freeborn TJ, Maundy B, Elwakil A (2011) Numerical extraction of Cole-Cole impedance parameters from step response. Nonlinear Theory Appl, IEICE 2: 548-561. doi: 10.1587/nolta.2.548
[13]	Freeborn TJ, Maundy B, Elwakil AS (2012) Least squares estimation technique of Cole-Cole parameters from step response. Electron Lett 48: 752-754. doi: 10.1049/el.2012.0360
[14]	Maundy BJ, Elwakil AS, Allagui A (2015) Extracting the parameters of the single-dispersion Cole bioimpedance model using a magnitude-only method. Comput Electron Agr 119: 153-157. doi: 10.1016/j.compag.2015.10.014
[15]	Freeborn TJ, Elwakil AS, Maundy B (2017) Variability of Cole-model bioimpedance parameters using magnitude-only measurements of apples from a two-electrode configuration. Int J Food Prop 20: S507-S519. doi: 10.1080/10942912.2017.1300810
[16]	Freeborn TJ, Elwakil A, Maundy B (2016) Electrode location impact on cole-impedance parameters using magnitude-only measurements.
[17]	Chowdhury A, Datta S, Bera TK, et al. (2018) Design and development of microcontroller based instrumentation for studying complex bioelectrical impedance of fruits using electrical impedance spectroscopy. J Food Process Eng 41: e12640. doi: 10.1111/jfpe.12640
[18]	Lingwood BE (2013) Bioelectrical impedance analysis for assessment of fluid status and body composition in neonates—the good, the bad and the unknown. Eur J Clin Nutr 67: S28-S33. doi: 10.1038/ejcn.2012.162
[19]	Kyle UG, Bosaeus I, De Lorenzo AD, et al. (2004) Bioelectrical impedance analysis—part I: review of principles and methods. Clin Nutr 23: 1226-1243. doi: 10.1016/j.clnu.2004.06.004
[20]	Juansah J, Budiastra IW, Dahlan K, et al. (2014) Electrical properties of garut citrus fruits at low alternating current signal and its correlation with physicochemical properties during maturation. Int J Food Prop 17: 1498-1517. doi: 10.1080/10942912.2012.723233
[21]	Dovancescu S, Saporito S, Herold IHF, et al. (2019) Monitoring thoracic fluid content using bioelectrical impedance spectroscopy and Cole modeling. J Electr Bioimpedance 8: 107-115. doi: 10.5617/jeb.5611
[22]	Arijit R, Pranay B, Abhishek M (2019) Identification of bioimpedance parameters for characterizing of tissue: a case study with apple tissue by ANOVA.
[23]	Cybulska J, Pieczywek PM, Zdunek A (2012) The effect of Ca²⁺ and cellular structure on apple firmness and acoustic emission. Eur Food Res Technol 235: 119-128. doi: 10.1007/s00217-012-1743-6
[24]	Gancarz M, Konstankiewicz K (2007) Changes of cellular structure of potato tuber parenchyma tissues during storage. Res Agr Eng 53: 75-78. doi: 10.17221/2118-RAE
[25]	Singh F, Katiyar VK, Singh BP (2015) Mathematical modeling to study influence of porosity on apple and potato during dehydration. J Food Sci Tech 52: 5442-5455. doi: 10.1007/s13197-014-1647-5

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