Cellular ATP release in the lung and airway

Satoru Ito; Kishio Furuya; Masahiro Sokabe; Yoshinori Hasegawa; Satoru Ito; Kishio Furuya; Masahiro Sokabe; Yoshinori Hasegawa

doi:10.3934/biophy.2016.4.571

AIMS Biophysics

2016, Volume 3, Issue 4: 571-584. doi: 10.3934/biophy.2016.4.571

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Review Topical Sections

Cellular ATP release in the lung and airway

1.
Department of Respiratory Medicine
2.
Mechanobiology Laboratory, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan

Received: 27 September 2016 Accepted: 21 November 2016 Published: 28 November 2016

Adenosine triphosphate (ATP) is a universal energy source synthesized by mitochondrial oxidative phosphorylation and cytosolic glycolysis and transported by the vesicular nucleotide transporter for storage in secretory vesicles. Extracellular ATP regulates physiological functions and homeostasis of the respiratory system and is associated with pathogenesis of respiratory diseases. Thus, modulation of ATP and purinergic signaling may be a novel therapeutic approach to pulmonary disease. ATP is released from alveolar epithelial cells, airway epithelial cells, airway smooth muscle cells, fibroblasts and endothelial cells in response to various chemical and mechanical stimuli. In addition to conductive pathways such as connexins and pannexins, vesicular exocytosis is involved in the mechanisms of ATP release from the cells. Imaging approaches enable us to visualize ATP release from not only cultured cells but also lung tissue ex vivo. Extracellular vesicles, exosomes and membrane-derived microvesicles, containing cytoplasmic proteins, mRNA and microRNA, represent important mediators of cell-to-cell communication and the intercellular microenvironment. However, it is not known whether extracellular vesicles contain ATP as an intercellular messenger. Future studies are necessary to elucidate the mechanisms of cellular ATP release and purinergic signaling in the respiratory system.
- airway smooth muscle,
- asthma,
- ATP release,
- COPD,
- endothelial cells,
- exocytosis,
- fibroblast,
- lung tissue,
- mechanotransduction,
- stretch
Citation: Satoru Ito, Kishio Furuya, Masahiro Sokabe, Yoshinori Hasegawa. Cellular ATP release in the lung and airway[J]. AIMS Biophysics, 2016, 3(4): 571-584. doi: 10.3934/biophy.2016.4.571

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Abstract

Adenosine triphosphate (ATP) is a universal energy source synthesized by mitochondrial oxidative phosphorylation and cytosolic glycolysis and transported by the vesicular nucleotide transporter for storage in secretory vesicles. Extracellular ATP regulates physiological functions and homeostasis of the respiratory system and is associated with pathogenesis of respiratory diseases. Thus, modulation of ATP and purinergic signaling may be a novel therapeutic approach to pulmonary disease. ATP is released from alveolar epithelial cells, airway epithelial cells, airway smooth muscle cells, fibroblasts and endothelial cells in response to various chemical and mechanical stimuli. In addition to conductive pathways such as connexins and pannexins, vesicular exocytosis is involved in the mechanisms of ATP release from the cells. Imaging approaches enable us to visualize ATP release from not only cultured cells but also lung tissue ex vivo. Extracellular vesicles, exosomes and membrane-derived microvesicles, containing cytoplasmic proteins, mRNA and microRNA, represent important mediators of cell-to-cell communication and the intercellular microenvironment. However, it is not known whether extracellular vesicles contain ATP as an intercellular messenger. Future studies are necessary to elucidate the mechanisms of cellular ATP release and purinergic signaling in the respiratory system.

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