Research article

Bio-active compounds, their antioxidant activities, and the physicochemical and pasting properties of both pigmented and non-pigmented fermented de-husked rice flour

  • Received: 23 August 2020 Accepted: 09 November 2020 Published: 01 December 2020
  • The aim of this study was to determine the effect of both the Solid State Fermentation (SSF) technique and the use of Rhizopus oligosporus on the physicochemical changes of fermented de-husked rice flour. Three varieties of de-husked rice, i.e., Mentik Wangi Susu (non-pigmented), red Cempo Merah, and black Jowo Melik (pigmented) were fermented using Rhizopus oligosporus. Fermentation was performed at room temperature with a fermentation time of 0, 24, 48, and 72 hours. The analyzed parameters were proximate composition, bio-active compounds, and pasting profile. The results showed an increase in flour pasting profile, ash, protein, and fat content after the fermentation. The total availability of the total phenolic content (TPC) and antioxidant capacity were also increased. The highest TPC (0.37 mg GAE/g) and antioxidant capacity (1.43 mg TEAC/g) were obtained in the Jowo Melik variety at 72 hours of fermentation. In contrast, anthocyanin and carbohydrate contents decreased as fermentation time increased. The highest anthocyanin content of 0.53 mg/g (after 24-hour fermentation) was obtained in the Jowo Melik variety. In conclusion, 72-hour-fermented black rice flour (Jowo Melik) has a higher potential to be developed as a functional food.

    Citation: Budi Suarti, Sukarno, Ardiansyah, Slamet Budijanto. Bio-active compounds, their antioxidant activities, and the physicochemical and pasting properties of both pigmented and non-pigmented fermented de-husked rice flour[J]. AIMS Agriculture and Food, 2021, 6(1): 49-64. doi: 10.3934/agrfood.2021004

    Related Papers:

  • The aim of this study was to determine the effect of both the Solid State Fermentation (SSF) technique and the use of Rhizopus oligosporus on the physicochemical changes of fermented de-husked rice flour. Three varieties of de-husked rice, i.e., Mentik Wangi Susu (non-pigmented), red Cempo Merah, and black Jowo Melik (pigmented) were fermented using Rhizopus oligosporus. Fermentation was performed at room temperature with a fermentation time of 0, 24, 48, and 72 hours. The analyzed parameters were proximate composition, bio-active compounds, and pasting profile. The results showed an increase in flour pasting profile, ash, protein, and fat content after the fermentation. The total availability of the total phenolic content (TPC) and antioxidant capacity were also increased. The highest TPC (0.37 mg GAE/g) and antioxidant capacity (1.43 mg TEAC/g) were obtained in the Jowo Melik variety at 72 hours of fermentation. In contrast, anthocyanin and carbohydrate contents decreased as fermentation time increased. The highest anthocyanin content of 0.53 mg/g (after 24-hour fermentation) was obtained in the Jowo Melik variety. In conclusion, 72-hour-fermented black rice flour (Jowo Melik) has a higher potential to be developed as a functional food.


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    [1] de Mira NVM, Massaretto IL, Pascual CDSCI, et al. (2009) Comparative study of phenolic compounds in different Brazilian rice (Oryza sativa L.) genotypes. J Food Compos Anal 22: 405-409.
    [2] Shao Y, Xu F, Sun X, et al. (2014) Identification and quantification of phenolic acids and anthocyanins as antioxidants in bran, embryo and endosperm of white, red and black rice kernels (Oryza sativa L.). J Cereal Sci 59: 211-218.
    [3] Gao Y, Guo X, Liu Y, et al. (2018) Comparative assessment of phytochemical profile, antioxidant capacity and antiproliferative activity in different varieties of brown rice (Oryza sativa L.). LWT 96: 19-25.
    [4] Razak DLA, Abd Rashid NY, Jamaluddin A, et al. (2015) Enhancement of phenolic acid content and antioxidant activity of rice bran fermented with Rhizopus oligosporus and Monascus purpureus. Biocatal Agric Biotechnol 4: 33-38.
    [5] Noviasari S, Kusnandar F, Setiyono A, et al. (2019) Profile of phenolic compounds, DPPH-scavenging and anti α-amylase activity of black rice bran fermented with Rhizopus oligosporus. Pertanika J Trop Agric Sci 42: 489-501.
    [6] Bhanja T, Kumari A, Banerjee R (2009) Bioresource technology enrichment of phenolics and free radical scavenging property of wheat koji prepared with two filamentous fungi. Bioresour Technol 100: 2861-2866.
    [7] Oliveiera M, Cipolatti EP, Badiale-furlong E, et al. (2012) Phenolic compounds and antioxidant activity in fermented rice (Oryza sativa) Phenolic compounds and antioxidant activity in fermented rice (Oryza sativa) bran. Food Sci Technol 32: 531-536.
    [8] Hayat A, Jahangir TM, Khuhawar MY, et al. (2015) HPLC determination of gamma amino butyric acid (GABA) and some biogenic amines (BAs) in controlled, germinated, and fermented brown rice by pre-column derivatization. J. Cereal Sci 64: 56-62.
    [9] AOAC (2015) Official methods of analysis of AOAC International 18th edition. AOAC International.
    [10] Giusti MM, Wrolstad RE (2001) Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy. Current Protocols in Food Analytical Chemistry. John Wiley & Sons, Inc., New York. DOI: 10.1002/0471142913.faf0102s00.
    [11] Reddy CKR, Imi LK, Aripriya SH, et al. (2017) Effects of polishing on proximate composition, physico- chemical characteristics, mineral composition and antioxidant properties of pigmented rice. Rice Sci 24: 241-252.
    [12] Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28: 25-30.
    [13] AACC (1999) AACC International Method. 61-03.01: Determination of the pasting properties of rice with the rapid visco analyzer. Minnesota (US): American Association of Cereal Chemists. 2-5.
    [14] Surojanametakul V, Panthavee W, Satmalee P, et al. (2019) Effect of traditional dried starter culture on morphological, chemical and physicochemical properties of sweet fermented glutinous rice products. J Agric Sci 11: 43-51.
    [15] Chinsamran K, Piyachomkwan K, Santisopasri V (2005) Effect of lactic acid fermentation on physico-chemical properties of starch derived from cassava, sweet potato and rice effect of lactic acid fermentation on physico-chemical properties of starch derived from cassava, sweet potato and rice. Kasetsart J Nat Sci 39: 76-87.
    [16] Chu J, Zhao H, Lu Z, et al. (2019) Improved physicochemical and functional properties of dietary fi ber from millet bran fermented by Bacillus natto. Food Chem 294: 79-86.
    [17] SNI (2009) Tepung Beras. Badan Standardisasi Nasional. Jakarta. Available from: https://bsn.go.id/uploads/download/skema_tepung_%E2%80%93_lampiran_xx_perka_bsn_11_tahun_2019.pdf.
    [18] Liang J, Han BZ, Nout MJR, et al. (2008) Effects of soaking, germination and fermentation on phytic acid, total and in vitro soluble zinc in brown rice. Food Chem 110: 821-828.
    [19] Liang J, Li Z, Tsuji K, Nakano K, et al. (2008) Milling characteristics and distribution of phytic acid and zinc in long-, medium- and short-grain rice. J. Cereal Sci 48: 83-91.
    [20] Iwai T, Takahashi M, Oda K, et al. (2014) Dynamic changes in the distribution of minerals in relation to phytic acid accumulation during rice. Plant Physiol 160: 2007-2014.
    [21] Suresh S, Radha KV (2015) Effect of a mixed substrate on phytase production by Rhizopus oligosporus MTCC 556 using solid state fermentation and determination of dephytinization activities in food grains. Food Sci Biotechnol 24: 551-559.
    [22] Oduguwa OO, Edema MO, Ayeni A (2008) Physico-chemical and microbiological analyses of fermented corn cob, rice bran and cowpea husk for use in composite rabbit feed. Bioresour Technol 99: 1816-1820.
    [23] Benabda O, Sana M, Kasmi M, et al. (2019) Optimization of protease and amylase production by Rhizopus oryzae cultivated on bread waste using solid-state Fermentation. J Chem 2019: 1-9.
    [24] Handoyo T, Morita N, (2006) Structural and functional properties of fermented soybean (tempeh) by using Rhizopus oligosporus. Int J Food Prop 9: 347-355.
    [25] Verma DK, Srivastav PP (2017) Proximate composition, mineral content and fatty acids analyses of aromatic and non-aromatic indian rice. Rice Sci 24: 21-31.
    [26] Oliveira S, Feddern V, Kupski L, et al. (2011) Bioresource technology changes in lipid, fatty acids and phospholipids composition of whole rice bran after solid-state fungal fermentation. Bioresour Technol 102: 8335-8338.
    [27] Oliveira MDS, Feddern V, Kupski L, et al. (2010) Physico-chemical characterization of fermented rice bran biomass[ Caracterización fisico-química de la biomasa del salvado de arroz fermentado]. CyTA-J Food 8: 229-236.
    [28] Ribeiro AC, Graca CS, Chiattoni ML, et al. (2017) Fermentation process in the availability of nutrients in rice bran. RR: J Microbiol Biotechnol 6: 45-52.
    [29] Kong EL, Lee BK, Michelle, et al. (2015) DNA damage inhibitory effect and phytochemicals of fermented red brown rice extract. Asian Pacific J Trop Dis 5: 732-736.
    [30] Schmidt CG, Gonç alves LM, Prietto L, et al. (2014) Antioxidant activity and enzyme inhibition of phenolic acids from fermented rice bran with fungus Rizhopus oryzae. Food Chem 146: 371-377.
    [31] Kumar P, Prakash KS, Jan K, et al. (2017) Effects of gamma irradiation on starch granule structure and physicochemical properties of brown rice starch. J Cereal Sci 77: 194-200.
    [32] Zhang MW, Zhang RF, Zhang FX, et al. (2010) Phenolic profiles and antioxidant activity of black rice bran of different commercially available varieties. J Agric Food Chem 58: 7580-7587.
    [33] Chaiyasut C, Pengkumsri N, Sirilun S, et al. (2017) Assessment of changes in the content of anthocyanins, phenolic acids, and antioxidant property of Saccharomyces cerevisiae mediated fermented black rice bran. AMB Expr 7: 114.
    [34] Abdel-Aal ESM, Young JC, Rabalski I (2019) Anthocyanin composition in black, blue, pink, purple, and red cereal grains. Agric Food Chem 54: 4696-4704.
    [35] Maulani RR, Sumardi D, Pancoro A (2019) Total flavonoids and anthocyanins content of pigmented rice. Drug Invent Today 12: 369-373.
    [36] Luximon-Ramma A, Bahorun T, Soobrattee M, et al. (2002) Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula. J Agric Food Chem 50: 5042-5047.
    [37] Cao G, Sofic E, Prior RL (1997) Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic Biol Med 22: 749-760.
    [38] Chen M, Meng H, Zhao Y, et al. (2015) Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses. BMC Complementary Altern Med 15: 313.
    [39] Anggraini T, Novelina, Limber U, et al. (2015) Antioxidant activities of some red, black and white rice cultivar from West Sumatra, Indonesia. Pak J Nutr 14: 112-117.
    [40] Butsat S, Siriamornpun S (2010) Antioxidant capacities and phenolic compounds of the husk, bran and endosperm of Thai rice. Food Chem 119: 606-613.
    [41] Pang Y, Ahmed S, Xu Y, et al. (2018) Bound phenolic compounds and antioxidant properties of whole grain and bran of white, red and black rice. Food Chem 240: 212-221.
    [42] Juhász R, Salgó A (2008) Pasting behavior of amylose, amylopectin and their mixtures as determined by rva curves and First Derivatives. Starch-Stärke 60: 70-78.
    [43] Patindol J, Wang YJ, Jane JL (2005) Structure-functionality changes in starch following rough rice storage. Starch-Stärke 57: 197-207.
    [44] Olanipekun BF, Otunola ET, Adelakun OE, et al. (2009) Effect of fermentation with Rhizopus oligosporus on some physico-chemical properties of starch extracts from soybean flour. Food Chem Toxicol 47: 1401-1405.
    [45] Balogun IO, Olatidoye OP, Otunola ET (2019) Effect of fermentation with R. oligosporus and R. stolonifer on some physicochemical properties of starch extracts from dehulled and undehulled. Int Res J Appl Sci 1: 71-75.
    [46] Ikegwu OJ, Okechukwu PE, Ekumankana EO (2010) Physico-chemical and pasting characteristic of flour and starch from achi Brachytegia eurycoms seed. J Food Technol 8: 58-66.
    [47] Varavinit S, Shobsngob S, Varanyanond W, et al. (2003) Effect of amylose content on gelatinization, retrogradation and pasting properties of flours from different cultivars of thai rice. Starch-Stärke 55: 410-415.
    [48] Oloyede OO, James S, Ocheme OB, et al. (2015) Effects of fermentation time on the functional and pasting properties of defatted Moringa oleifera seed flour. Food Sci Nutr 4: 89-95.
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