Ethanol production at high temperature from cassava pulp by a newly isolated <em>Kluyveromyces marxianus</em> strain, TISTR 5925

Waraporn Apiwatanapiwat; Prapassorn Rugthaworn; Pilanee Vaithanomsat; Warunee Thanapase; Akihiko Kosugi; Takamitsu Arai; Yutaka Mori; Yoshinori Murata; Waraporn Apiwatanapiwat; Prapassorn Rugthaworn; Pilanee Vaithanomsat; Warunee Thanapase; Akihiko Kosugi; Takamitsu Arai; Yutaka Mori; Yoshinori Murata

doi:10.3934/energy.2013.1.3

AIMS Energy

2013, Volume 1, Issue 1: 3-16. doi: 10.3934/energy.2013.1.3

Previous Article Next Article

Research article

Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925

1.
Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;
2.
Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50 Chatuchak, Bangkok 10900, Thailand

Received: 25 July 2013 Accepted: 09 October 2013 Published: 31 October 2013

Kluyveromyces marxianus TISTR 5925, isolated from rotten fruit in Thailand, can ferment at pH 3 at temperatures between 42 and 45 ℃. Bioethanol production from cassava pulp using the simultaneous saccharification and fermentation (SSF) process was evaluated and compared with the separated hydrolysis and fermentation (SHF) process using K. marxianus TISTR 5925. The ethanol concentrations obtained from the SSF process were higher than those from the SHF process. The optimum conditions for ethanol production were investigated by response surface methodology (RSM) based on a five level central composite design involving the following variables: enzyme dilution (times), temperature (℃) and fermentation time (h). Cassava pulp was pretreated by boiling for 10 min, treated with a mixture of enzymes (cellulase, pectinase, α-amylase and glucoamylase), then fermented by K. marxianus TISTR 5925. Data obtained from the RSM were subjected to analysis of variance and fit to a second order polynomial equation. At optimum enzyme dilution (0.1 times), temperature (41 ℃) and fermentation time (27 h), the maximum obtained concentration of ethanol was 5.0% (w/v), which is very close to the predicted ethanol concentration of 5.3% (w/v).
- Ethanol,
- Thermotolerant,
- Response surface methodology,
- Cassava pulp
Citation: Waraporn Apiwatanapiwat, Prapassorn Rugthaworn, Pilanee Vaithanomsat, Warunee Thanapase, Akihiko Kosugi, Takamitsu Arai, Yutaka Mori, Yoshinori Murata. Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925[J]. AIMS Energy, 2013, 1(1): 3-16. doi: 10.3934/energy.2013.1.3

Related Papers:

Abstract

Kluyveromyces marxianus TISTR 5925, isolated from rotten fruit in Thailand, can ferment at pH 3 at temperatures between 42 and 45 ℃. Bioethanol production from cassava pulp using the simultaneous saccharification and fermentation (SSF) process was evaluated and compared with the separated hydrolysis and fermentation (SHF) process using K. marxianus TISTR 5925. The ethanol concentrations obtained from the SSF process were higher than those from the SHF process. The optimum conditions for ethanol production were investigated by response surface methodology (RSM) based on a five level central composite design involving the following variables: enzyme dilution (times), temperature (℃) and fermentation time (h). Cassava pulp was pretreated by boiling for 10 min, treated with a mixture of enzymes (cellulase, pectinase, α-amylase and glucoamylase), then fermented by K. marxianus TISTR 5925. Data obtained from the RSM were subjected to analysis of variance and fit to a second order polynomial equation. At optimum enzyme dilution (0.1 times), temperature (41 ℃) and fermentation time (27 h), the maximum obtained concentration of ethanol was 5.0% (w/v), which is very close to the predicted ethanol concentration of 5.3% (w/v).

References

[1]	Gray, KA.; Zhao, L.; Emptage, M. et al. (2006) Bioethanol: a review. Curr Opin Chem Biol 10: 141-146. doi: 10.1016/j.cbpa.2006.02.035
[2]	El-Sharkawy, MA. Cassava biology and physiology. (2004) Plant Mol Biol 56: 481-501.
[3]	Sorapipatana, C.; Yoosin, S. (2011) Life cycle cost of ethanol production from cassava in Thailand. Renew Sust Energ Rev 15: 1343-1349. doi: 10.1016/j.rser.2010.10.013
[4]	Sriroth, K.; Chollakup, R.; Chotineeranat, S.; Piyachomkwan, K.; Oates, CG. et al. (2000) Processing of cassava waste for improved biomass utilization. Bioresour Technol 71: 63-69. doi: 10.1016/S0960-8524(99)00051-6
[5]	Kosugi, A.; Kondo, A.; Ueda, M.; Murata, Y.; Vaithanomsat, P.; Thanapase, W. et al. (2009) Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase. Renew Ener 34: 1354-1358. doi: 10.1016/j.renene.2008.09.002
[6]	Rattanachomsri, U.; Tanapongpipat, S.; Eurwilaichitr, L.; Champreda, V. et al. (2009) Simultaneous non-thermal saccharification of cassava pulp by multi-enzyme activity and ethanol fermentation by Candida tropicalis. J Biosc Bioeng 107: 488-493. doi: 10.1016/j.jbiosc.2008.12.024
[7]	Apiwatanapiwat, W.; Murata, Y.; Kosugi, A.; Yamada, R.; Kondo, A.; Arai, T. et al. (2011) Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase. Appl. Microbiol Biotechnol 90: 377-384. doi: 10.1007/s00253-011-3115-8
[8]	Abdel-Banat, BM.; Hoshida, H.; Ano, A.; Nonklang, S.; Akada, R. et al. (2009) High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast? Appl Microbiol Biotechnol 85: 861-867.
[9]	Limtong, S.; Sringiew, C.; Yongmanitchai, W. et al. (2007) Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus. Bioresour Technol 98: 3367-3374. doi: 10.1016/j.biortech.2006.10.044
[10]	Anderson, PJ.; McNeil, K.; Watson, K. et al. (1986) High-efficiency carbohydrate fermentation to ethanol at temperatures above 40℃ by Kluyveromyces marxianus var. marxianus isolated from sugar mills. Appl Environ Microbiol 51: 1314-1320.
[11]	Nonklang, S.; Abdel-Banat, BM.; Cha-aim, K.; Moonjai, N.; Hoshida, H.; Limtong, S. et al. (2008) High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042. Appl Environ Microbiol 74: 7514-7521. doi: 10.1128/AEM.01854-08
[12]	Fonseca, GG.; Heinzle, E.; Wittmann, C.; Gombert, AK. et al. (2008) The yeast Kluyveromyces marxianus and its biotechnological potential. Appl Microb Biotechnol 79: 339-354. doi: 10.1007/s00253-008-1458-6
[13]	Chen, QH.; He, GQ.; Ali, MAM. et al. (2002) Optimization of medium composition for the production of elastase by Bacillus sp. EL31410 with response surface methodology. Enzyme Microb Technol 30: 667-672.
[14]	Altschul, SF.; Gish, W.; Miller, W.; Meyers, EW.; Lipman, DJ. et al. (1990) Basic local 129 alignment search tool. J Mol Biol 215: 403-410. doi: 10.1016/S0022-2836(05)80360-2
[15]	Somogyi, M. (1952) Notes in Sugar Determination. J Biol Chem 195: 19-23.
[16]	Sreekumar, O.; Chand, N.; Basappa, SC. et al. (1999) Optimization and interaction of media components in ethanol production using Zymomonas mobilis by response surface methodology. J Biosci Bioeng 88: 334-338. doi: 10.1016/S1389-1723(00)80021-3
[17]	Manikandan, K.; Viruthagiri, T. (2010) Optimization of C/N ratio of the medium and fermentation conditions of ethanol production from tapioca starch using co-culture of Aspergillus niger and Sacharomyces cerevisiae. International Journal of Chem. Tech Research 2: 947-955.
[18]	Ubalue, AO. (2007) Cassava wastes: treatment options and value addition alternatives: a review. Afri J Biotechnol 6: 2065-2073.
[19]	Srichuwong, S.; Fujiwara, M.; Wang, X.; Seyama, T.; Shiroma, R.; Arakane, M. et al. (2009) Simultaneous saccharification and fermentation (SSF) of very high gravity (VHG) potato mash for the production of ethanol. Biomass Bioenerg 33: 890-986. doi: 10.1016/j.biombioe.2009.01.012
[20]	Olofsson, K.; Bertilsson, M.; Lidén, G. et al. (2008) A short review on SSF - an interesting process option for ethanol production from lignocellulosic feedstocks: a review. Biotechnol. Biofuels 1: 1-14. doi: 10.1186/1754-6834-1-1
[21]	Sasikumar, E.; Viruthagiri, T. (2010) Simultaneous saccharification and fermentation (SSF) ofsugarcane bagasse-kinetics and modeling. International Journal of Chemical and Biological Engineering 3: 57-64.

Reader Comments

Your name:*

Email:*
© 2013 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)