Association of interleukin-2 gene variants (positions +114 and −384) and susceptibility to brucellosis in Iranian population

Sohrab Najafipour; Abdolreza karami; Manoochehr Rasouli; Mehdi Kalani; Seyed-Jalal-Aldin Ashraf-Mansoori; Ali Moravej; Sohrab Najafipour; Abdolreza karami; Manoochehr Rasouli; Mehdi Kalani; Seyed-Jalal-Aldin Ashraf-Mansoori; Ali Moravej

doi:10.3934/molsci.2017.1.103

AIMS Molecular Science

2017, Volume 4, Issue 1: 103-109. doi: 10.3934/molsci.2017.1.103

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Association of interleukin-2 gene variants (positions +114 and −384) and susceptibility to brucellosis in Iranian population

1.
Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran
2.
Department of Microbiology, Jahrom Islamic Azad University, Jahrom, Iran
3.
Department of Immunology, Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Received: 29 December 2016 Accepted: 06 March 2017 Published: 13 March 2017

Brucella is an obligate intracellular gram negative bacterium and the causative agent of brucellosis. Interleukin-2 (IL-2) is a cytokine involved in cell-mediated immunity response secreted by activated T-cells and considered as the growth factor for T-cells. Previous reports have revealed that gene polymorphisms of cytokines can affect susceptibility to Brucella infection. The goal of this study was to investigate the relationship between IL-2 gene polymorphisms (positions +114 and −384) and susceptibility to brucellosis. A total of 173 brucellosis patients and 75 healthy animal husbandmen who had Brucella infected animals and consumed their contaminated dairy products, as control group, were included in this study. All participants were genotyped for IL-2 gene polymorphisms at positions +114 (G/T) and −384 (G/T), using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The frequency of TT genotype at the position +114 was significantly higher in the controls, compared with the patients. But, there was no significant difference between the groups regarding TG and GG genotypes as well as T and G alleles. Furthermore, at position −384, the frequencies of G allele and GG genotype were higher in the controls compared with patients, however, they were not significantly different Additionally, TT/TT haplogenotype (+114/−384) was significantly higher in the controls, compared with the patients. Conclusively, it is suggested that the inheritance of TT genotype (position +114) and TT/TT haplogenotype (+114/−384) of IL-2 gene could be considered as one of the genetic factors responsible for resistance to brucellosis.
- IL-2,
- polymorphism,
- Brucella,
- allele,
- genotype
Citation: Sohrab Najafipour, Abdolreza karami, Manoochehr Rasouli, Mehdi Kalani, Seyed-Jalal-Aldin Ashraf-Mansoori, Ali Moravej. Association of interleukin-2 gene variants (positions +114 and −384) and susceptibility to brucellosis in Iranian population[J]. AIMS Molecular Science, 2017, 4(1): 103-109. doi: 10.3934/molsci.2017.1.103

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Abstract

Brucella is an obligate intracellular gram negative bacterium and the causative agent of brucellosis. Interleukin-2 (IL-2) is a cytokine involved in cell-mediated immunity response secreted by activated T-cells and considered as the growth factor for T-cells. Previous reports have revealed that gene polymorphisms of cytokines can affect susceptibility to Brucella infection. The goal of this study was to investigate the relationship between IL-2 gene polymorphisms (positions +114 and −384) and susceptibility to brucellosis. A total of 173 brucellosis patients and 75 healthy animal husbandmen who had Brucella infected animals and consumed their contaminated dairy products, as control group, were included in this study. All participants were genotyped for IL-2 gene polymorphisms at positions +114 (G/T) and −384 (G/T), using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The frequency of TT genotype at the position +114 was significantly higher in the controls, compared with the patients. But, there was no significant difference between the groups regarding TG and GG genotypes as well as T and G alleles. Furthermore, at position −384, the frequencies of G allele and GG genotype were higher in the controls compared with patients, however, they were not significantly different Additionally, TT/TT haplogenotype (+114/−384) was significantly higher in the controls, compared with the patients. Conclusively, it is suggested that the inheritance of TT genotype (position +114) and TT/TT haplogenotype (+114/−384) of IL-2 gene could be considered as one of the genetic factors responsible for resistance to brucellosis.

References

[1]	Corbel MJ (1997) Brucellosis: an overview. Emerg Infect Dis 3: 213-321. doi: 10.3201/eid0302.970219
[2]	Mantur BG, Mangalgi SS, Mulimani M (1996) Brucella melitensis: Asexually transmissible agent? Lancet 347: 1763.
[3]	Eckman MR (1975) Brucellosis linked to Mexican cheese. JAMA 232: 636-637. doi: 10.1001/jama.1975.03250060032018
[4]	Malik GM (1997) A clinical study of brucellosis in adult in the Asir region of southern Saudi Arabia. Am J Trop Med Hyg 56: 375-377.
[5]	Glass WI (1970) Brucellosis as an occupational disease in New Zealand. N Z Med J 63: 301-308.
[6]	William E (1970) Brucellosis and the British public. Lancet 1: 1220-1222.
[7]	Pappas G, Akritidis N, Bosilkovski M, et al. (2005) Brucellosis. N Engl J Med 352: 2325-2336. doi: 10.1056/NEJMra050570
[8]	Kozukeev TB, Ajeilat S, Maes E, et al. (2006) Risk factors for brucellosis--Leylek and Kadamjay districts, Batken Oblast, Kyrgyzstan, January-November, 2003. MMWR Suppl 28: 31-34.9. Refik M, Mehmet N, Duramz R, et al. (2004) Cytokine profile and nitric oxide levels in sera from patients with Brucellosis. Braz J Med Biol Res 37: 20.
[9]	10. Bassat H, Saadany S, Yamany A, et al. (2005) Role of T-helper-1 Cytokine and Nitric oxide production in patients with Acute Brucellosis. Tanta Med 3: 1087-10.
[10]	11. Kariminia A, Kavossy G, Khatami S, et al. (2002) Study of interleukin-and interleukin-12 productions in response to lipopolysaccharides extracted from two different Brucella strains. Comp Immunol Microbiol Infect Dis 25: 85-93. doi: 10.1016/S0147-9571(01)00029-7
[11]	12. Zhan Y, Cheers C (1995) Endogenous interleukin-12 is involved in resistance to Brucella abortus infection. Infect Immun 63: 1387-1390.
[12]	13. Hoover DL, Crawford RM, Van De Verg LL, et al. (1999) Protection of mice against bruellosis by vaccination with Brucella melittensis WR201 (16M A Pur EK). Infect Immun 67:5877-5884.
[13]	14. Pasqual P, Adone R, Gasbarre LC, et al. (2001) mouse cytokine profiles associated with Brucella abortus RB51 vaccination or B. abortus 2308 infection. Infect Immun 69: 6541-6544.
[14]	15. Serre A, Bascoul S, Vendrell JP, et al. (1987) Human immune response to Brucella infection. Ann Inst Pasteur Microbiol 138: 113-117.
[15]	16. Rasouli M, Kiany S (2007) Association of interferon-gamma and interleukin-4 gene polymorphisms with susceptibility to Brucellosis in Iranian patients. Cytokine 38: 49-53. doi: 10.1016/j.cyto.2007.05.003
[16]	17. Rasouli M, Kiany S, Behbin M (2008) Interleukin-10 Gene Polymorphisms and Susceptibility to Brucellosis in Iranian patients. Iran J Immunol 5: 131-135.
[17]	18. Rasouli M, Kiany S, Moravej A, et al. (2010) Interleukin-12 and Tumor Necrosis Factor- Gene Polymorphisms as Genetic Susceptibility Factors for Brucellosis in Iranian Patients. Iran Red Crescent Med J 12: 266-271.
[18]	19. Matesanz F, Fedetz M, Collado-Romero M, et al. (2001) Allelic expression and interleukin-2 polymorphisms in multiple sclerosis. J Neuroimmunol 119: 101-105. doi: 10.1016/S0165-5728(01)00354-X
[19]	20. Wu HC, Chang CH, Tsai FJ, et al. (2006) IL-2 gene C/T polymorphisms is associated with prostate Cancer. J Clin Lab Anal 20: 425-429.
[20]	21. Peng Q, Li H, Lao X, et al. (4) Association of IL-2 polymorphisms and IL-2 serum levels with susceptibility to HBV-related hepatocellular carcinoma in a Chinese Zhuang population. Infect Genet Evol 27: 375-381. doi: 10.1016/j.meegid.2014.08.021

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