How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of <em>Cannabis sativa</em> stem tissues

Lauralie Mangeot-Peter; Sylvain Legay; Jean-Francois Hausman; Gea Guerriero; Lauralie Mangeot-Peter; Sylvain Legay; Jean-Francois Hausman; Gea Guerriero

doi:10.3934/molsci.2016.4.560

AIMS Molecular Science

2016, Volume 3, Issue 4: 560-566. doi: 10.3934/molsci.2016.4.560

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How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of Cannabis sativa stem tissues

Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), L-4362 Esch/Alzette, Luxembourg

Received: 07 July 2016 Accepted: 26 September 2016 Published: 28 September 2016

The preservation of intact RNA is a limiting step when gene expression profiling is performed using field-collected plant material. The use of liquid nitrogen ensures the optimal preservation of RNA, however it is not always practical, especially if the plant material has to be sampled in remote locations. Ethanol is known to preserve DNA in plant tissues even after a long storage period and here its suitability to preserve the RNA of textile hemp cortical tissues was tested. Hemp (Cannabis sativa L.) is an economically important fibre crop because it supplies cellulosic bast fibres used in different industrial sectors. In this study we demonstrate the suitability of ethanol for RNA preservation by analyzing tissues stored at 4 °C for 1, 2, 4 and 8 days. We show that in all the cases the extracted RNA is intact. We finally analyze hemp stem tissues stored in ethanol for 1 month and demonstrate the preservation of the tissue structure, particularly of bast fibres.
- RNA preservation,
- ethanol,
- bast fibres,
- Cannabis sativa,
- integrity
Citation: Lauralie Mangeot-Peter, Sylvain Legay, Jean-Francois Hausman, Gea Guerriero. How to store plant tissues in the absence of liquid nitrogen? Ethanol preserves the RNA integrity of Cannabis sativa stem tissues[J]. AIMS Molecular Science, 2016, 3(4): 560-566. doi: 10.3934/molsci.2016.4.560

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Abstract

The preservation of intact RNA is a limiting step when gene expression profiling is performed using field-collected plant material. The use of liquid nitrogen ensures the optimal preservation of RNA, however it is not always practical, especially if the plant material has to be sampled in remote locations. Ethanol is known to preserve DNA in plant tissues even after a long storage period and here its suitability to preserve the RNA of textile hemp cortical tissues was tested. Hemp (Cannabis sativa L.) is an economically important fibre crop because it supplies cellulosic bast fibres used in different industrial sectors. In this study we demonstrate the suitability of ethanol for RNA preservation by analyzing tissues stored at 4 °C for 1, 2, 4 and 8 days. We show that in all the cases the extracted RNA is intact. We finally analyze hemp stem tissues stored in ethanol for 1 month and demonstrate the preservation of the tissue structure, particularly of bast fibres.

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