The surface nanostructures of titanium alloy regulate the proliferation of endothelial cells

Min Lai; Xiaofang Yang; Qing Liu; Jinghua Li; Yanhua Hou; Xiuyong Chen; Kaiyong Cai; Min Lai; Xiaofang Yang; Qing Liu; Jinghua Li; Yanhua Hou; Xiuyong Chen; Kaiyong Cai

doi:10.3934/matersci.2014.1.45

AIMS Materials Science

2014, Volume 1, Issue 1: 45-58. doi: 10.3934/matersci.2014.1.45

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Research article

The surface nanostructures of titanium alloy regulate the proliferation of endothelial cells

1.
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China;
2.
School of Materials Science and Engineering, Chongqing University, Chongqing 400044, China

Received: 04 December 2013 Accepted: 26 January 2014 Published: 09 February 2014

To investigate the effect of surface nanostructures on the behaviors of human umbilical vein endothelial cells (HUVECs), surface nanostructured titanium alloy (Ti-3Zr2Sn-3Mo-25Nb, TLM) was fabricated by surface mechanical attrition treatment (SMAT) technique. Field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were employed to characterize the surface nanostructures of the TLM, respectively. The results demonstrated that nano-crystalline structures with several tens of nanometers were formed on the surface of TLM substrates. The HUVECs grown onto the surface nanostructured TLM spread well and expressed more vinculin around the edges of cells. More importantly, HUVECs grown onto the surface nanostructured TLM displayed significantly higher (p < 0.01 or p < 0.05) cell adhesion and viabilities than those of native titanium alloy. HUVECs cultured on the surface nanostructured titanium alloy displayed significantly higher (p < 0.01 or p < 0.05) productions of nitric oxide (NO) and prostacyclin (PGI2) than those of native titanium alloy, respectively. This study provides an alternative for the development of titanium alloy based vascular stents.
- titanium alloy,
- human umbilical vein endothelial cells,
- SMAT,
- nanostructure,
- cell behavior
Citation: Min Lai, Xiaofang Yang, Qing Liu, Jinghua Li, Yanhua Hou, Xiuyong Chen, Kaiyong Cai. The surface nanostructures of titanium alloy regulate the proliferation of endothelial cells[J]. AIMS Materials Science, 2014, 1(1): 45-58. doi: 10.3934/matersci.2014.1.45

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Abstract

To investigate the effect of surface nanostructures on the behaviors of human umbilical vein endothelial cells (HUVECs), surface nanostructured titanium alloy (Ti-3Zr2Sn-3Mo-25Nb, TLM) was fabricated by surface mechanical attrition treatment (SMAT) technique. Field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were employed to characterize the surface nanostructures of the TLM, respectively. The results demonstrated that nano-crystalline structures with several tens of nanometers were formed on the surface of TLM substrates. The HUVECs grown onto the surface nanostructured TLM spread well and expressed more vinculin around the edges of cells. More importantly, HUVECs grown onto the surface nanostructured TLM displayed significantly higher (p < 0.01 or p < 0.05) cell adhesion and viabilities than those of native titanium alloy. HUVECs cultured on the surface nanostructured titanium alloy displayed significantly higher (p < 0.01 or p < 0.05) productions of nitric oxide (NO) and prostacyclin (PGI2) than those of native titanium alloy, respectively. This study provides an alternative for the development of titanium alloy based vascular stents.

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