Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation

M Thirumala; E Sai Krishna; P Sindhu Priya; S Vishnuvardhan Reddy; M Thirumala; E Sai Krishna; P Sindhu Priya; S Vishnuvardhan Reddy

doi:10.3934/microbiol.2022031

AIMS Microbiology

2022, Volume 8, Issue 4: 470-483. doi: 10.3934/microbiol.2022031

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Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation

1.
Microbial Ecology Laboratory, Department of Biochemistry, UCS, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda-508254, Telangana, India
2.
Microbztech Labs Pvt. Ltd., Cherlapally, Nalgonda-508001, Telangana, India

Received: 17 August 2022 Revised: 10 November 2022 Accepted: 15 November 2022 Published: 23 November 2022

A Gram positive rod shaped bacterium designated as isolate H1 with Fluoride resistance up to 4 g/L sodium fluoride (NaF) in LB (Luria-Bertani) agar was isolated from a ground water sample of Narketpally area, Nalgonda district, Telangana, India. The colonies of isolate H1 were off white in color. Growth patterns of isolate H1 were observed at two different concentrations, 100 and 250 ppm, of NaF and also without NaF in the medium. In cases where NaF was present in the media, the lag phases of the growth curves were extended when compared to the absence of NaF. Optimum pH required for the organism's growth was 8. Isolate H1 required a temperature of 37 °C with 150 rpm and 2% NaCl for its optimal growth in the medium without NaF. Meanwhile, isolate H1 could thrive in a diverse pH range, i.e., pH 5–10, and at an NaCl concentration of up to 11% in the medium with NaF. Based on morphological, biochemical and molecular characterization, isolate H1 was identified as belonging to the genus Bacillus. It showed 98.47% 16S rDNA gene sequence similarity with Bacillus australimaris NH71_1^T. Isolate H1 showed high fluoride removals of 22.5% and 38.2% with 100 and 250 mg/L of NaF in the LB broth when incubated at pH 8 and a temperature of 37 °C with 150 rpm for 3 day. Hence, this organism could be a promising isolate to apply for defluoridation of ground water in fluoride contaminthe ated areas.
- defluoridation,
- fluoride resistance,
- Bacillus sp.,
- sodium fluoride,
- 16S rDNA
Citation: M Thirumala, E Sai Krishna, P Sindhu Priya, S Vishnuvardhan Reddy. Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation[J]. AIMS Microbiology, 2022, 8(4): 470-483. doi: 10.3934/microbiol.2022031

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Abstract

A Gram positive rod shaped bacterium designated as isolate H1 with Fluoride resistance up to 4 g/L sodium fluoride (NaF) in LB (Luria-Bertani) agar was isolated from a ground water sample of Narketpally area, Nalgonda district, Telangana, India. The colonies of isolate H1 were off white in color. Growth patterns of isolate H1 were observed at two different concentrations, 100 and 250 ppm, of NaF and also without NaF in the medium. In cases where NaF was present in the media, the lag phases of the growth curves were extended when compared to the absence of NaF. Optimum pH required for the organism's growth was 8. Isolate H1 required a temperature of 37 °C with 150 rpm and 2% NaCl for its optimal growth in the medium without NaF. Meanwhile, isolate H1 could thrive in a diverse pH range, i.e., pH 5–10, and at an NaCl concentration of up to 11% in the medium with NaF. Based on morphological, biochemical and molecular characterization, isolate H1 was identified as belonging to the genus Bacillus. It showed 98.47% 16S rDNA gene sequence similarity with Bacillus australimaris NH71_1^T. Isolate H1 showed high fluoride removals of 22.5% and 38.2% with 100 and 250 mg/L of NaF in the LB broth when incubated at pH 8 and a temperature of 37 °C with 150 rpm for 3 day. Hence, this organism could be a promising isolate to apply for defluoridation of ground water in fluoride contaminthe ated areas.

Acknowledgments

Authors are thankful to the people of Nalgonda district for their support during the field study. Authors are also thankful to Dr. M. Anjaneyulu, Incharge HoD, Dept. of Geology, MGU for helping with Fluoride analysis.

Conflict of interest

The authors disclosed no potential conflicts of interest, financial or otherwise.

Authors' contributions

MT and SVR were involved in the conception and design of the work, data analysis and drafting of the article. SKE and SPP were involved in experimental works and data collection. MT and SVR revised and approved the final version of the manuscript.

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