Tannic and gallic acids alter redox-parameters of the medium and modulate biofilm formation

Zoya Samoilova; Alexey Tyulenev; Nadezhda Muzyka; Galina Smirnova; Oleg Oktyabrsky; Zoya Samoilova; Alexey Tyulenev; Nadezhda Muzyka; Galina Smirnova; Oleg Oktyabrsky

doi:10.3934/microbiol.2019.4.379

AIMS Microbiology

2019, Volume 5, Issue 4: 379-392. doi: 10.3934/microbiol.2019.4.379

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Research article Special Issues

Tannic and gallic acids alter redox-parameters of the medium and modulate biofilm formation

Laboratory of Physiology and Genetics of Microorganisms, Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, Perm Federal Research Center, Perm, Russia

Received: 15 October 2019 Accepted: 09 December 2019 Published: 27 December 2019

Tannic (TA) and gallic (GA) acids are known to have both anti- and prooxidant properties however recently they have been described as potential anti-biofilm agents although their mechanisms of action on bacterial cells remain obscure. The aim of our research was to elucidate the role of prooxidant actions of these plant phenolic compounds in bactericidal effects and biofilm formation. In our experiments, both compounds demonstrated strong oxidative properties that altered activity of stress regulons and contributed to decrease of CFU and ability of cells to maintain membrane potential. Stimulation of biofilm formation was observed in all the strains with the exception of the strains deficient in flagella synthesis. Both compounds demonstrated bactericidal effect which was weakened in biofilms. TA efficiently killed bacteria in the bioflms of pgaA mutant which pointed out an important role of poly-beta-1,6-N-acetyl-D-glucosamine (PGA) polysaccharide in matrix formation. Similar effects of TA in recA mutant indicate involvement of SOS-response into reaction towards exposure with TA. Gallic acid-induced killing was more pronounced in the biofilms of csgA mutant revealing role of curli in protection against GA toxicity.
- tannic and gallic acid,
- mutations in biofilm formation,
- pgaA,
- csgA,
- SOS-response,
- Escherichia coli
Citation: Zoya Samoilova, Alexey Tyulenev, Nadezhda Muzyka, Galina Smirnova, Oleg Oktyabrsky. Tannic and gallic acids alter redox-parameters of the medium and modulate biofilm formation[J]. AIMS Microbiology, 2019, 5(4): 379-392. doi: 10.3934/microbiol.2019.4.379

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Abstract

Tannic (TA) and gallic (GA) acids are known to have both anti- and prooxidant properties however recently they have been described as potential anti-biofilm agents although their mechanisms of action on bacterial cells remain obscure. The aim of our research was to elucidate the role of prooxidant actions of these plant phenolic compounds in bactericidal effects and biofilm formation. In our experiments, both compounds demonstrated strong oxidative properties that altered activity of stress regulons and contributed to decrease of CFU and ability of cells to maintain membrane potential. Stimulation of biofilm formation was observed in all the strains with the exception of the strains deficient in flagella synthesis. Both compounds demonstrated bactericidal effect which was weakened in biofilms. TA efficiently killed bacteria in the bioflms of pgaA mutant which pointed out an important role of poly-beta-1,6-N-acetyl-D-glucosamine (PGA) polysaccharide in matrix formation. Similar effects of TA in recA mutant indicate involvement of SOS-response into reaction towards exposure with TA. Gallic acid-induced killing was more pronounced in the biofilms of csgA mutant revealing role of curli in protection against GA toxicity.

Acknowledgments

This work was supported by state assignment 01201353246, also by the grant of Russian Foundation of Basic Research RFBR-Ural 19-44-590009 and by the grant from President of Russian Federation for young scientists MK-3376.2018.4.

Conflict of interest

The authors declare that they have no conflict of interest.

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