Development of sample preparation method for organochlorine pesticides analysis in soil samples

Thanawat Jumepaeng; Nirand Pongpun; Thanawat Jumepaeng; Nirand Pongpun

doi:10.3934/environsci.2020025

AIMS Environmental Science

2020, Volume 7, Issue 5: 380-386. doi: 10.3934/environsci.2020025

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Research article

Development of sample preparation method for organochlorine pesticides analysis in soil samples

Thanawat Jumepaeng ^{1
,
,},
Nirand Pongpun ²

1.
Faculty of Sports Science and Health, Thailand National Sports University Udon Thani Campus, Udon Thani, Thailand
2.
Faculty of Science, Srinakharinwirot University, Bangkok, Thailand

Received: 23 August 2020 Accepted: 29 September 2020 Published: 30 September 2020

OCPs are the persistent organic pollutants which are highly toxic to the environment and can accumulate in humans by foods such as rice, vegetable, and fruits. The aim of this research was to develop a rapid and easy sample preparation method for determination 4 OCPs, aldrin, dieldrin, β-endosulfane, and p-p' DDT in soil samples by gas chromatography with electron capture detection. The analysis was performed by using HP-5 capillary column with the isothermal condition at 250 oC and carrier gas flow rate of 1 mL/min. The results show that the 4 OCPs and internal standard, pentachloronitrobenzene, were analyzed within 6 min. The optimized condition of the sample preparation method was using ultrasonic assisted solvent extraction with 20 mL of hexane and ethyl acetate (9:1 v/v) as an extractant for 15x2 min. The results obtained from the proposed sample preparation method have demonstrated that the limit of detection was 0.628–3.68 μg kg^-1. Percent recoveries were in the range of 81.42 to 110.7% and RSD were 1.68–9.43%. Correlation coefficients of calibration graphs were more than 0.99. The developed sample preparation method exhibited simplicity rapid and low chemical reagent consumption with satisfactory results.
- none clean-up method,
- ultrasonic extraction,
- Thai soil sample,
- electron capture detector
Citation: Thanawat Jumepaeng, Nirand Pongpun. Development of sample preparation method for organochlorine pesticides analysis in soil samples[J]. AIMS Environmental Science, 2020, 7(5): 380-386. doi: 10.3934/environsci.2020025

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Abstract

OCPs are the persistent organic pollutants which are highly toxic to the environment and can accumulate in humans by foods such as rice, vegetable, and fruits. The aim of this research was to develop a rapid and easy sample preparation method for determination 4 OCPs, aldrin, dieldrin, β-endosulfane, and p-p' DDT in soil samples by gas chromatography with electron capture detection. The analysis was performed by using HP-5 capillary column with the isothermal condition at 250 oC and carrier gas flow rate of 1 mL/min. The results show that the 4 OCPs and internal standard, pentachloronitrobenzene, were analyzed within 6 min. The optimized condition of the sample preparation method was using ultrasonic assisted solvent extraction with 20 mL of hexane and ethyl acetate (9:1 v/v) as an extractant for 15x2 min. The results obtained from the proposed sample preparation method have demonstrated that the limit of detection was 0.628–3.68 μg kg^-1. Percent recoveries were in the range of 81.42 to 110.7% and RSD were 1.68–9.43%. Correlation coefficients of calibration graphs were more than 0.99. The developed sample preparation method exhibited simplicity rapid and low chemical reagent consumption with satisfactory results.

References

[1]	Chung S, Chen B (2011) Determination of organochlorine pesticide residues in fatty foods: A critical review on the analytical methods and their testing capabilities. J Chromatogr A 1218: 5555-5567.
[2]	Fernandes V, Pestana D, Monteiro R, et al. (2012) Optimization and validation of organochlorine compounds in adipose tissue by SPE‐gas chromatography. Biomed Chromatogr 26: 1494-1501.
[3]	Zhang N, Yang Y, Tao S, et al. (2011) Sequestration of organochlorine pesticides in soils of distinct organic carbon content. Environ Pollut 159: 700-705.
[4]	Rissato S, Galhiane M, Apon B, et al. (2005) Multiresidue Analysis of pesticides in soil by supercritical fluid extraction/gas chromatography with electron-capture detection and confirmation by gas chromatography−mass spectrometry. J Agric Food Chem 53: 62-69.
[5]	Stoytcheva M (2011) Pesticides—strategies for pesticides analysis. London Intechopen.
[6]	Fatoki O, Awofolu R (2003) Methods for selective determination of persistent organochlorine pesticide residues in water and sediments by capillary gas chromatography and electron-capture detection. J Chromatogr A 983: 225-236.
[7]	Chunzhou D, Zhaorui Z, Ming Y (2005) Determination of organochlorine pesticides and their derivations in water after HS-SPME using polymethyl-phenylvinylsiloxane-coated fiber by GC-ECD. Water Res 39: 4204-4210.
[8]	Dabrowska H, Dabrowski L, Biziuk M, et al. (2003) Solid-phase extraction clean-up of soil and sediment extracts for the determination of various types of pollutants in a single run. J Chromatogr A 1003: 29-42.
[9]	Tor A, Mehmet A, Senar O (2006) Ultrasonic solvent extraction of organochlorine pesticides from soil. Anal Chim Acta 559: 173-180.

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