Effective approaches for modulating the evolution of cheese quality attributes are needed for mitigating challenges that are associated with fluctuating supply and demand as well as with disrupt supply chain. Proteolysis is the most important and most complex cascade of events that affects the evolution of cheese quality attributes. Information about the effects of small changes in temperature during isothermal and non-isothermal aging of Cheddar cheese at temperatures lower than 10 ℃ on proteolysis has been developed to a very limited extent. The objective of the research was to age FF and RF Cheddar cheeses for six months at different isothermal and non-isothermal time-at-temperature regimes at temperature ranging from 5 to 8 ℃ and to investigate the effects of these conditions on proteolysis. Changes in the level of cheese-N fractions that are soluble at pH 4.6, soluble in 12% TCA and soluble in 5% PTA were monitored. The proteolytic cascade during aging was significantly (p < 0.05) influenced by a combined impact of the time-at-temperature details of aging and cheese composition. The highest and lowest levels of the investigated fractions were found in cheeses that had been aged isothermally at 8 and 5 ℃, respectively. In most cases, proteolysis in the FF cheeses was to a higher extent than in the RF ones. Proteolysis during non-isothermal aging was significantly affected by the aging regime in a time-at-temperature-specific manner (p < 0.05). The results can offer new opportunities for modulating the rate of cheese aging. The demonstrated significant effect of a very small change in aging temperature on proteolysis during cheese aging also highlights the critical importance of establishing and maintaining isotropic temperature distribution in cheese aging rooms.
Citation: Moshe Rosenberg, Yael Rosenberg. The effects of small changes in temperature on proteolysis during isothermal and non-isothermal aging of full-fat and reduced-fat Cheddar cheese[J]. AIMS Agriculture and Food, 2022, 7(2): 341-356. doi: 10.3934/agrfood.2022022
Effective approaches for modulating the evolution of cheese quality attributes are needed for mitigating challenges that are associated with fluctuating supply and demand as well as with disrupt supply chain. Proteolysis is the most important and most complex cascade of events that affects the evolution of cheese quality attributes. Information about the effects of small changes in temperature during isothermal and non-isothermal aging of Cheddar cheese at temperatures lower than 10 ℃ on proteolysis has been developed to a very limited extent. The objective of the research was to age FF and RF Cheddar cheeses for six months at different isothermal and non-isothermal time-at-temperature regimes at temperature ranging from 5 to 8 ℃ and to investigate the effects of these conditions on proteolysis. Changes in the level of cheese-N fractions that are soluble at pH 4.6, soluble in 12% TCA and soluble in 5% PTA were monitored. The proteolytic cascade during aging was significantly (p < 0.05) influenced by a combined impact of the time-at-temperature details of aging and cheese composition. The highest and lowest levels of the investigated fractions were found in cheeses that had been aged isothermally at 8 and 5 ℃, respectively. In most cases, proteolysis in the FF cheeses was to a higher extent than in the RF ones. Proteolysis during non-isothermal aging was significantly affected by the aging regime in a time-at-temperature-specific manner (p < 0.05). The results can offer new opportunities for modulating the rate of cheese aging. The demonstrated significant effect of a very small change in aging temperature on proteolysis during cheese aging also highlights the critical importance of establishing and maintaining isotropic temperature distribution in cheese aging rooms.
| [1] | Beresford T, Williams A (2004) The microbiology of cheese ripening. In: Cheese: Chemistry, Physics and Microbiology, 1st Edition, 287-317. https://doi.org/10.1016/S1874-558X(04)80071-X |
| [2] | Upadhyay VK, McSweeney PLH, Magboul AAA, et al. (2004) Proteolysis in cheese during ripening. In: Cheese: Chemistry, Physics and Microbiology, 1st Edition, 391-433, VⅢ. https://doi.org/10.1016/S1874-558X(04)80076-9 |
| [3] | Fox PF, Guinee TP, Cogan TM, et al. (2016) Microbiology of cheese ripening. In: Fundamentals of Cheese Science, Springer, Boston, MA., US., 333-390. https://doi.org/10.1007/978-1-4899-7681-9_11 |
| [4] |
Khattab AR, Guirguis HA, Tawfik SM, et al. (2019) Cheese ripening: A review on modern technologies towards flavor enhancement, process acceleration and improved quality assessment. Trends Food Sci Technol 88: 343-360. https://doi.org/10.1016/j.tifs.2019.03.009 doi: 10.1016/j.tifs.2019.03.009
|
| [5] | Ardö Y, McSweeney PLH, Magboul AAA, et al. (2017) Biochemistry of cheese ripening: Proteolysis. In: Cheese: Chemistry, Physics and Microbiology, 4th Edition, 445-482. https://doi.org/10.1016/B978-0-12-417012-4.00018-1 |
| [6] | Law BA (2010) Cheese-ripening and cheese flavour technology. In: Technology of Cheesemaking, 2nd Edition, 231-259. https://doi.org/10.1002/9781444323740.ch7 |
| [7] |
Sousa MJ, Ardö Y, McSweeney PLH (2001) Advances in the study of proteolysis during cheese ripening. Int Dairy J 11: 327-345. https://doi.org/10.1016/S0958-6946(01)00062-0 doi: 10.1016/S0958-6946(01)00062-0
|
| [8] |
Fox PF, McSweeney PLH (1996) Proteolysis in cheese during ripening. Food Rev Int 12: 457-509. https://doi.org/10.1080/87559129609541091 doi: 10.1080/87559129609541091
|
| [9] |
Fenelon MA, Guinee TP (2000) Primary proteolysis and textural changes during ripening in Cheddar cheeses manufactured to different fat contents. Int Dairy J 10: 151-158. https://doi.org/10.1016/S0958-6946(00)00040-6 doi: 10.1016/S0958-6946(00)00040-6
|
| [10] | Curtin Á C, McSweeney PLH (2004) Catabolism of amino acids in cheese during ripening. In: Fox PF, McSweeney PLH, Cogan TM, et al. (Eds.), Cheese: Chemistry, Physics and Microbiology: Academic Press, 435-454. https: //doi.org/10.1016/S1874-558X(04)80077-0 |
| [11] |
Williams AG, Noble J, Banks JM (2001) Catabolism of amino acids by lactic acid bacteria isolated from Cheddar cheese. Int Dairy J 11: 203-215. https://doi.org/10.1016/S0958-6946(01)00050-4 doi: 10.1016/S0958-6946(01)00050-4
|
| [12] | Luo J, Wang Y, Li B, et al. (2018) Effect of somatic cells composition on proteolysis and quality of Cheddar cheese. Trans Chin Soc Agric Eng 34: 282-288. |
| [13] |
Marino R, Considine T, Sevi A, et al. (2005) Contribution of proteolytic activity associated with somatic cells in milk to cheese ripening. Int Dairy J 15: 1026-1033. https://doi.org/10.1016/j.idairyj.2004.10.006 doi: 10.1016/j.idairyj.2004.10.006
|
| [14] |
McCarthy CM, Wilkinson MG, Guinee TP (2017) Effect of coagulant type and level on the properties of half-salt, half-fat Cheddar cheese made with or without adjunct starter: Improving texture and functionality. Int Dairy J 75: 30-40. https://doi.org/10.1016/j.idairyj.2017.07.006 doi: 10.1016/j.idairyj.2017.07.006
|
| [15] |
Milesi MM, McSweeney PLH, Hynes ER (2008) Impact of chymosin- and plasmin-mediated primary proteolysis on the growth and biochemical activities of lactobacilli in miniature cheddar-type cheeses. J Dairy Sci 91: 3277-3290. https://doi.org/10.3168/jds.2008-1197 doi: 10.3168/jds.2008-1197
|
| [16] |
Mø ller KK, Rattray FP, Hø ier E, et al. (2012) Manufacture and biochemical characteristics during ripening of Cheddar cheese with variable NaCl and equal moisture content. Dairy Sci Technol 92: 515-540. https://doi.org/10.1007/s13594-012-0076-3 doi: 10.1007/s13594-012-0076-3
|
| [17] |
O'Mahony JA, Lucey JA, McSweeney PLH (2005) Chymosin-mediated proteolysis, calcium solubilization, and texture development during the ripening of Cheddar cheese. J Dairy Sci 88: 3101-3114. https://doi.org/10.3168/jds.S0022-0302(05)72992-1 doi: 10.3168/jds.S0022-0302(05)72992-1
|
| [18] |
Paludetti LF, O'Callaghan TF, Sheehan JJ, et al. (2020) Effect of Pseudomonas fluorescens proteases on the quality of Cheddar cheese. J Dairy Sci 103: 7865-7878. https://doi.org/10.3168/jds.2019-18043 doi: 10.3168/jds.2019-18043
|
| [19] |
McCarthy CM, Wilkinson MG, Kelly PM, et al. (2016) Effect of salt and fat reduction on proteolysis, rheology and cooking properties of Cheddar cheese. Int Dairy J 56: 74-86. https://doi.org/10.1016/j.idairyj.2016.01.001 doi: 10.1016/j.idairyj.2016.01.001
|
| [20] |
McCarthy CM, Wilkinson MG, Kelly PM, et al. (2015) Effect of salt and fat reduction on the composition, lactose metabolism, water activity and microbiology of Cheddar cheese. Dairy Sci Technol 95: 587-611. https://doi.org/10.1007/s13594-015-0245-2 doi: 10.1007/s13594-015-0245-2
|
| [21] |
Soodam K, Ong L, Powell IB, et al. (2017) Effect of elevated temperature on the microstructure of full fat Cheddar cheese during ripening. Food Struct 14: 8-16. https://doi.org/10.1016/j.foostr.2017.05.003 doi: 10.1016/j.foostr.2017.05.003
|
| [22] | Aston JW, Fedrick IA, Durward IG, et al. (1983) The effect of elevated ripening temperatures on proteolysis and flavour development in Cheddar cheese. 1. Higher initial storage temperatures. N Z J Dairy Sci Technol 18: 143-151. |
| [23] |
Folkertsma B, Fox PF, McSweeney PLH (1996) Accelerated ripening of Cheddar cheese at elevated temperatures. Int Dairy J 6: 1117-1134. https://doi.org/10.1016/0958-6946(95)00066-6 doi: 10.1016/0958-6946(95)00066-6
|
| [24] |
Sihufe GA, Zorrilla SE, Perotti MC, et al. (2010) Acceleration of cheese ripening at elevated temperature. An estimation of the optimal ripening time of a traditional Argentinean hard cheese. Food Chem 119: 101-107. https://doi.org/10.1016/j.foodchem.2009.06.001 doi: 10.1016/j.foodchem.2009.06.001
|
| [25] |
Azarnia S, Robert N, Lee BH (2006) Biotechnological methods to accelerate cheddar cheese ripening. Crit Rev Biotechnol 26: 121-143. https://doi.org/10.1080/07388550600840525 doi: 10.1080/07388550600840525
|
| [26] |
Aston JW, Giles JE, Durward IG, et al. (1985) Effect of elevated ripening temperatures on proteolysis and flavour development in Cheddar cheese. J Dairy Res 52: 565-572. https://doi.org/10.1017/S0022029900024523 doi: 10.1017/S0022029900024523
|
| [27] |
Walsh EA, Diako C, Smith DM, et al. (2020) Influence of storage time and elevated ripening temperature on the chemical and sensory properties of white Cheddar cheese. J Food Sci 85: 268-278. https://doi.org/10.1111/1750-3841.14998 doi: 10.1111/1750-3841.14998
|
| [28] |
Fox PF, Wallace JM, Morgan S, et al. (1996) Acceleration of cheese ripening. Antonie van Leeuwenhoek 70: 271-297. https://doi.org/10.1007/BF00395937 doi: 10.1007/BF00395937
|
| [29] |
Fenelon MA, Ryan MP, Rea MC, et al. (1999) Elevated temperature ripening of reduced fat Cheddar made with or without Lacticin 3147-producing starter culture. J Dairy Sci 82: 10-22. https://doi.org/10.3168/jds.S0022-0302(99)75203-3 doi: 10.3168/jds.S0022-0302(99)75203-3
|
| [30] |
Law BA (2001) Controlled and accelerated cheese ripening: the research base for new technology. Int Dairy J 11: 383-398. https://doi.org/10.1016/S0958-6946(01)00067-X doi: 10.1016/S0958-6946(01)00067-X
|
| [31] |
Hannon JA, Wilkinson MG, Delahunty CM, et al. (2005) Application of descriptive sensory analysis and key chemical indices to assess the impact of elevated ripening temperatures on the acceleration of Cheddar cheese ripening. Int Dairy J 15: 263-273. https://doi.org/10.1016/j.idairyj.2004.08.001 doi: 10.1016/j.idairyj.2004.08.001
|
| [32] | Hooi R, Barbano DM, Bradley RL, et al. (2004) Chapter 15: Chemical and physical methods. In: Arnold EA, (Eds.), Standard Methods for the Examination of Dairy Products, American Public Health Association. https: //doi.org/10.2105/9780875530024ch15 |
| [33] |
Altemueller AG, Rosenberg M (1996) Monitoring proteolysis during ripening of full-fat and low-fat Cheddar cheeses by Reverse-Phase HPLC. J Food Sci 61: 295-298. https://doi.org/10.1111/j.1365-2621.1996.tb14179.x doi: 10.1111/j.1365-2621.1996.tb14179.x
|
| [34] | Kuchroo CN, Fox PF (1982) Soluble nitrogen in Cheddar cheese: comparison of extraction procedures. Milchwissenschaft-Milk Sci Int 37: 331-335. |
| [35] |
McSweeney PLH, Fox PF (1997) Chemical methods for the characterization of proteolysis in cheese during ripening. Lait 77: 41-76. https://doi.org/10.1051/lait:199713 doi: 10.1051/lait:199713
|
| [36] |
Rosenberg M, Altemueller A (2001) Accumulation of free L-Glutamic Acid in full- and reduced-fat Cheddar cheese ripened at different time/temperature conditions. LWT-Food Sci Technol 34: 279-287. https://doi.org/10.1006/fstl.2001.0754 doi: 10.1006/fstl.2001.0754
|
| [37] | Frister H, Michaelis M, Schwerdtfeger T, et al. (2000) Evaluation of bitterness in Cheddar cheese. Milchwissenschaft 55: 691-695. |
| [38] | Banks J, Weimer B (2007) Chapter: 23: Producing low fat cheese. In: Weimer BC, (Eds.), Improving the Flavour of Cheese, Woodhead Publishing, 520-536. https: //doi.org/10.1533/9781845693053.4.520 |
| [39] |
Fenelon MA, O'Connor P, Guinee TP (2000) The effect of fat content on the microbiology and proteolysis in Cheddar cheese during ripening. J Dairy Sci 83: 2173-2183. https://doi.org/10.3168/jds.S0022-0302(00)75100-9 doi: 10.3168/jds.S0022-0302(00)75100-9
|
| [40] |
Kenny O, FitzGerald RJ, O'Cuinn G, et al. (2006) Autolysis of selected Lactobacillus helveticus adjunct strains during Cheddar cheese ripening. Int Dairy J 16: 797-804. https://doi.org/10.1016/j.idairyj.2005.07.008 doi: 10.1016/j.idairyj.2005.07.008
|
| [41] |
Hu Y, Ge K, Jiang L, et al. (2013) Effect of Transglutaminase on yield, compositional and functional properties of low-fat Cheddar cheese. Food Sci Technol Res 19: 359-367. https://doi.org/10.3136/fstr.19.359 doi: 10.3136/fstr.19.359
|
| [42] | Kiernan RC, Beresford TP, O'Cuinn G, et al. (2000) Autolysis of Lactobacilli during Cheddar cheese ripening. Ir J Agric Food Res 39: 95-106. |
Moshe Rosenberg, Yael Rosenberg. The effects of small changes in temperature on proteolysis during isothermal and non-isothermal aging of full-fat and reduced-fat Cheddar cheese[J]. AIMS Agriculture and Food, 2022, 7(2): 341-356. doi: 10.3934/agrfood.2022022
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