Preventive effect of <i>Enterococcus faecalis</i> and β-glucan on the onset and exacerbation of symptoms of atopic dermatitis in mice

Kazunobu Baba; Akina Oomori; Tadaaki Miyazaki; Kazunobu Baba; Akina Oomori; Tadaaki Miyazaki

doi:10.3934/Allergy.2023013

AIMS Allergy and Immunology

2023, Volume 7, Issue 3: 183-194. doi: 10.3934/Allergy.2023013

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Research article

Preventive effect of Enterococcus faecalis and β-glucan on the onset and exacerbation of symptoms of atopic dermatitis in mice

Department of Synbiotics, Institute for Genetic Medicine, North15 West7, Kitaku, Sapporo, Hokkaido, Japan

Received: 14 April 2023 Revised: 04 June 2023 Accepted: 19 June 2023 Published: 06 July 2023

Atopic dermatitis (AD) is associated with an immune response caused by the excessive production of IgE antibodies. This is accompanied by excessive itching as a clinical symptom, leading to a great burden and anxiety in daily life. At present, there is no established treatment that can completely cure AD, and symptomatic treatment alleviates symptoms to the extent that they do not interfere with daily life. Recent research has suggested that the ingestion of either various lactic acid bacteria or β-glucan suppressed the production of IgE antibodies and alleviated the symptoms of AD. Therefore, in this study, we used a drug-induced AD mouse model to evaluate the amelioration effect of EC-12 and β-glucan derived from black yeast to investigate the mechanism involved. The oral administration of lactic acid bacteria inhibited the expression of inflammatory cytokine genes at the lesion site, and the administration of β-glucan downregulated serum IgE levels and inhibited the expression of inflammatory cytokine genes at the lesion site. The concomitant administration of EC-12 and β-glucan strongly suppressed AD symptoms, downregulated serum IgE levels, and inhibited the expression of inflammatory cytokines at the lesion site. In summary, the oral administration of EC-12 and β-glucan prevented AD in mice.
- synbiotics,
- β-glucan,
- Enterococcus faecalis,
- aureobasidium pullulans,
- atopic dermatitis
Citation: Kazunobu Baba, Akina Oomori, Tadaaki Miyazaki. Preventive effect of Enterococcus faecalis and β-glucan on the onset and exacerbation of symptoms of atopic dermatitis in mice[J]. AIMS Allergy and Immunology, 2023, 7(3): 183-194. doi: 10.3934/Allergy.2023013

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Abstract

Atopic dermatitis (AD) is associated with an immune response caused by the excessive production of IgE antibodies. This is accompanied by excessive itching as a clinical symptom, leading to a great burden and anxiety in daily life. At present, there is no established treatment that can completely cure AD, and symptomatic treatment alleviates symptoms to the extent that they do not interfere with daily life. Recent research has suggested that the ingestion of either various lactic acid bacteria or β-glucan suppressed the production of IgE antibodies and alleviated the symptoms of AD. Therefore, in this study, we used a drug-induced AD mouse model to evaluate the amelioration effect of EC-12 and β-glucan derived from black yeast to investigate the mechanism involved. The oral administration of lactic acid bacteria inhibited the expression of inflammatory cytokine genes at the lesion site, and the administration of β-glucan downregulated serum IgE levels and inhibited the expression of inflammatory cytokine genes at the lesion site. The concomitant administration of EC-12 and β-glucan strongly suppressed AD symptoms, downregulated serum IgE levels, and inhibited the expression of inflammatory cytokines at the lesion site. In summary, the oral administration of EC-12 and β-glucan prevented AD in mice.

Abbreviations

atopic dermatitis

dendritic cells

DNFB

2,4-dinitrofluorobenzene

ELISA

enzyme-linked immuno-sorbent assay

glucocorticoid receptor

Acknowledgments

We would like to thank to the Representative Director and President, Atsuhiro Inaba in INABA Foods Co., Ltd. for the research grant and great help.

Use of AI tools declaration

The authors declare they have not used Artificial Intelligence (AI) tools in the creation of this article.

Conflict of interest

The authors declare no conflicts of interest associated with this manuscript.

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