Stress induced «railway for pre-ribosome export» structure as a new model for studying eukaryote ribosome biogenesis

E.N. Baranova; R.M. Sarimov; A.A. Gulevich; E.N. Baranova; R.M. Sarimov; A.A. Gulevich

doi:10.3934/biophy.2019.2.47

AIMS Biophysics

2019, Volume 6, Issue 2: 47-67. doi: 10.3934/biophy.2019.2.47

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Stress induced «railway for pre-ribosome export» structure as a new model for studying eukaryote ribosome biogenesis

1.
All-Russia Research Institute of Agricultural Biotechnology, Timiriazevskaya 42, 127550 Moscow, Russia
2.
Prokhorov General Physics Institute of the Russian Academy of Sciences, GPI RAS, 38 Vavilov str., 119991 Moscow, Russia
3.
Institute of Plant Physiology of the Russian Academy of Sciences, Moscow, Russia

Received: 03 April 2019 Accepted: 01 July 2019 Published: 18 July 2019

The article presents the details of the process of structural organization, formation and disassembly of the nucleolus, chromatin condensation during the passage of the cell cycle studied by the method of classical transmission electron microscopy (TEM) in the cells of the meristematic zone of the wheat root. The transformations of peripheral chromosome material (PCM) in telophase, the transformations of the nucleolar nucleolonema in the interphase and prophase, and the chromatin transformations (condensation and decondensation) were studied and analyzed under the conditions of the geomagnetic field, weakened magnetic field and static magnetic field. Using an analysis of the dynamics of structural transformations of proliferating nuclei, we observed a disturbance in the formation of the chromonema and condensed chromatin in the middle interphase, a change in the structural organization of the nucleolus during the transition from late interphase to prophase, as well as a change in the structure of PCM, pre-nucleolus and chromatin in telophase. A schema for the generation of a specific conformation of nucleolonema and chromonema and its influence on the structural organization of chromosomes in anaphase and telophase was proposed. The nature of this structure is discussed. Possible mechanisms of the effect of magnetic field on the nuclear compartment, regulation of chromatin decompactization, export of pre-ribosomal particles under stress, and the fine structure of the nucleolonema are considered.
- weakened magnetic field,
- cold,
- chromonema,
- nucleolonema,
- nucleolus,
- ribosome biogenesis
Citation: E.N. Baranova, R.M. Sarimov, A.A. Gulevich. Stress induced «railway for pre-ribosome export» structure as a new model for studying eukaryote ribosome biogenesis[J]. AIMS Biophysics, 2019, 6(2): 47-67. doi: 10.3934/biophy.2019.2.47

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Abstract

The article presents the details of the process of structural organization, formation and disassembly of the nucleolus, chromatin condensation during the passage of the cell cycle studied by the method of classical transmission electron microscopy (TEM) in the cells of the meristematic zone of the wheat root. The transformations of peripheral chromosome material (PCM) in telophase, the transformations of the nucleolar nucleolonema in the interphase and prophase, and the chromatin transformations (condensation and decondensation) were studied and analyzed under the conditions of the geomagnetic field, weakened magnetic field and static magnetic field. Using an analysis of the dynamics of structural transformations of proliferating nuclei, we observed a disturbance in the formation of the chromonema and condensed chromatin in the middle interphase, a change in the structural organization of the nucleolus during the transition from late interphase to prophase, as well as a change in the structure of PCM, pre-nucleolus and chromatin in telophase. A schema for the generation of a specific conformation of nucleolonema and chromonema and its influence on the structural organization of chromosomes in anaphase and telophase was proposed. The nature of this structure is discussed. Possible mechanisms of the effect of magnetic field on the nuclear compartment, regulation of chromatin decompactization, export of pre-ribosomal particles under stress, and the fine structure of the nucleolonema are considered.

Acknowledgments

The research was performed on the state assignment AAAA-A18-118051890089-0.

Conflict of interest

The authors declare no competing financial interests.

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