Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina

Madhura Kulkarni-Chitnis; Leah Mitchell-Bush; Remmington Belford; Jenaye Robinson; Catherine A. Opere; Sunny E. Ohia; Ya Fatou N. Mbye; Madhura Kulkarni-Chitnis; Leah Mitchell-Bush; Remmington Belford; Jenaye Robinson; Catherine A. Opere; Sunny E. Ohia; Ya Fatou N. Mbye

doi:10.3934/Neuroscience.2019.3.104

AIMS Neuroscience

2019, Volume 6, Issue 3: 104-115. doi: 10.3934/Neuroscience.2019.3.104

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Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina

1.
Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX 77004, USA
2.
Department of Pharmacy Sciences, School of Pharmacy and Health Professions, Creighton University, Omaha, NE 68178, USA

Received: 12 February 2019 Accepted: 28 May 2019 Published: 18 June 2019

Purpose: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H₂S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H₂S in bovine isolated neural retina. Methods: Isolated bovine neural retina were homogenized and tissue homogenates were treated with a NO synthase inhibitor, NO donor, heme oxygenase-1 inhibitor, and/donor. H₂S concentrations in bovine retinal homogenates were measured using a well-established colorimetric assay. Results: L-NAME (300 nM–500 μM) caused a concentration-dependent decrease in basal endogenous levels of H₂S by 86.2%. On the other hand, SNP (10–300 μM) elicited a concentration-related increase in H₂S levels from 18.3 nM/mg of protein to 65.7 nM/mg of protein. ZnPP-IX (300 nM–10 μM) caused a concentration-dependent increase in the endogenous production of H₂S whereas hemin (300 nM–20 μM) attenuated the basal levels of H₂S. Conclusion: We conclude that changes in the biosynthesis and availability of both NO and CO can interfere with the pathway/s involved in the production of H₂S in the retina. The demonstrated ability of NO, CO and H₂S to interact in the mammalian retina affirms a physiological/pharmacological role for these gaseous mediators in the eye.
- gasotransmitters,
- retina,
- endogenous production,
- hydrogen sulfide,
- carbon monoxide,
- nitric oxide
Citation: Madhura Kulkarni-Chitnis, Leah Mitchell-Bush, Remmington Belford, Jenaye Robinson, Catherine A. Opere, Sunny E. Ohia, Ya Fatou N. Mbye. Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina[J]. AIMS Neuroscience, 2019, 6(3): 104-115. doi: 10.3934/Neuroscience.2019.3.104

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Abstract

Purpose: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H₂S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H₂S in bovine isolated neural retina. Methods: Isolated bovine neural retina were homogenized and tissue homogenates were treated with a NO synthase inhibitor, NO donor, heme oxygenase-1 inhibitor, and/donor. H₂S concentrations in bovine retinal homogenates were measured using a well-established colorimetric assay. Results: L-NAME (300 nM–500 μM) caused a concentration-dependent decrease in basal endogenous levels of H₂S by 86.2%. On the other hand, SNP (10–300 μM) elicited a concentration-related increase in H₂S levels from 18.3 nM/mg of protein to 65.7 nM/mg of protein. ZnPP-IX (300 nM–10 μM) caused a concentration-dependent increase in the endogenous production of H₂S whereas hemin (300 nM–20 μM) attenuated the basal levels of H₂S. Conclusion: We conclude that changes in the biosynthesis and availability of both NO and CO can interfere with the pathway/s involved in the production of H₂S in the retina. The demonstrated ability of NO, CO and H₂S to interact in the mammalian retina affirms a physiological/pharmacological role for these gaseous mediators in the eye.

Abbreviation HS: Hydrogen sulfide; CO: Carbon monoxide; NO: Nitric oxide; cAMP: cyclic AMP; CSE: cystathionine γ-lyase; CBS: cystathionine β-synthase; L-NAME: L-nitroarginine methyl ester; SNP: sodium nitroprusside; ZnPP-IX: zinc protoporphyrin; PAG: propargyglycine; AOAA: aminooxyacetic acid;
Acknowledgments

This work was in part supported by National Institutes of Health/National Eye Institute Grant R15EY022215-01.
The authors would also like to acknowledge the Molecular Biology Research infrastructure support from grant number 2G12MD007605 from the NIMHD/NIH”NIMHD/NIH” 2G12MD007605.

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the manuscript. The manuscript has been read and approved by all contributing authors.

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