Enzyme-instructed self-assembly of peptides: Process, dynamics, nanostructures, and biomedical applications

Yun Chen; Bin Liu; Lei Guo; Zhong Xiong; Gang Wei; Yun Chen; Bin Liu; Lei Guo; Zhong Xiong; Gang Wei

doi:10.3934/biophy.2020028

AIMS Biophysics

2020, Volume 7, Issue 4: 411-428. doi: 10.3934/biophy.2020028

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Enzyme-instructed self-assembly of peptides: Process, dynamics, nanostructures, and biomedical applications

Yun Chen ¹,
Bin Liu ¹,
Lei Guo ²,
Zhong Xiong ^{1
,
,},
Gang Wei ^{1
,
,}

1.
College of Chemistry and Chemical Engineering, Qingdao University, 266071 Qingdao, PR China
2.
Research Center for High-Value Utilization of Waste Biomass, College of Life Science, Qingdao University, 266071 Qingdao, PR China

Received: 13 August 2020 Accepted: 26 September 2020 Published: 29 September 2020

Biomolecular self-assembly provides a potential way for the design and synthesis of functional biomaterials with uniform structure and unique properties, in which the self-assembly of peptide molecules is especially important ascribed to the controllable structural design and functional tailoring of peptide motifs. The self-assembly of peptides can be instructed by internal and external physical, chemical, and biological stimulations. Compared to both physical and chemical stimulations including light/thermal treatment, pH/ionic strength adjustment, and others, the biological mediation with enzymes exhibited great advantages due to its high bioactivity, excellent biocompatibility, high specificity, and in vivo reaction. Herein we summarize the advance in the enzyme-instructed peptide self-assembly for biomedical applications. For this aim, we introduce and discuss the self-assembly of peptide that controlled by both kinetics and dynamics, and then demonstrate the enzyme-induced preparation of peptide nanostructures such as nanofibers, nanotubes, vesicles, networks, and hydrogels. Finally, the biomedical applications of enzyme-induced self-assembled peptide nanomaterials for cancer diagnostics, cancer therapy, bioelectronic devices, and biosensors are presented. It is expected this work will inspire more studies on the using of bioactive enzymes for triggering the self-assembly of peptides to create various novel bionanomaterials, which could extend this interesting research field to others such as tissue engineering, biocatalysis, energy storage, and environmental science.
- peptide,
- self-assembly,
- enzyme,
- nanomaterials,
- biomedical applications
Citation: Yun Chen, Bin Liu, Lei Guo, Zhong Xiong, Gang Wei. Enzyme-instructed self-assembly of peptides: Process, dynamics, nanostructures, and biomedical applications[J]. AIMS Biophysics, 2020, 7(4): 411-428. doi: 10.3934/biophy.2020028

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Abstract

Biomolecular self-assembly provides a potential way for the design and synthesis of functional biomaterials with uniform structure and unique properties, in which the self-assembly of peptide molecules is especially important ascribed to the controllable structural design and functional tailoring of peptide motifs. The self-assembly of peptides can be instructed by internal and external physical, chemical, and biological stimulations. Compared to both physical and chemical stimulations including light/thermal treatment, pH/ionic strength adjustment, and others, the biological mediation with enzymes exhibited great advantages due to its high bioactivity, excellent biocompatibility, high specificity, and in vivo reaction. Herein we summarize the advance in the enzyme-instructed peptide self-assembly for biomedical applications. For this aim, we introduce and discuss the self-assembly of peptide that controlled by both kinetics and dynamics, and then demonstrate the enzyme-induced preparation of peptide nanostructures such as nanofibers, nanotubes, vesicles, networks, and hydrogels. Finally, the biomedical applications of enzyme-induced self-assembled peptide nanomaterials for cancer diagnostics, cancer therapy, bioelectronic devices, and biosensors are presented. It is expected this work will inspire more studies on the using of bioactive enzymes for triggering the self-assembly of peptides to create various novel bionanomaterials, which could extend this interesting research field to others such as tissue engineering, biocatalysis, energy storage, and environmental science.

Acknowledgments

The authors thank the financial support from the National Natural Science Foundation of China (No. 51873225), the High-Grade Talents Plan of Qingdao University, and the Taishan Scholars Program of Shandong Province (No. tsqn201909104).

Conflict of interest

The authors declared no conflict of interests.

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