Combinatorial pathway assembly in yeast

Khalil Essani; Anton Glieder; Martina Geier; Khalil Essani; Anton Glieder; Martina Geier

doi:10.3934/bioeng.2015.4.423

AIMS Bioengineering

2015, Volume 2, Issue 4: 423-436. doi: 10.3934/bioeng.2015.4.423

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Combinatorial pathway assembly in yeast

1.
Austrian Centre of Industrial Biotechnology (ACIB GmbH), 8010 Graz, Austria;
2.
Management Center Innsbruck (MCI), 6020 Innsbruck, Austria;
3.
Institute of Molecular Biotechnology, Graz University of Technology, 8010 Graz, Austria

Received: 14 June 2015 Accepted: 15 October 2015 Published: 21 October 2015

With the emergence of synthetic biology and the vast knowledge about individual biocatalytic reactions, the challenge nowadays is to implement whole natural or synthetic pathways into microorganisms. For this purpose balanced enzyme activities throughout the pathway need to be achieved in addition to simple functional gene expression to avoid bottlenecks and to obtain high titers of the desired product. As the optimization of pathways in a specific biological context is often hard to achieve by rational design, combinatorial approaches have been developed to address this issue. Here, current strategies and proof of concepts for combinatorial pathway assembly in yeasts are reviewed. By exploiting its ability to join multiple DNA fragments in a very efficient and easy manner, the yeast Saccharomyces cerevisiae does not only constitute an attractive host for heterologous pathway expression, but also for assembling pathways by recombination in vivo.
- heterologous pathway expression,
- homologous recombination,
- metabolic engineering,
- Saccharomyces cerevisiae,
- synthetic biology
Citation: Khalil Essani, Anton Glieder, Martina Geier. Combinatorial pathway assembly in yeast[J]. AIMS Bioengineering, 2015, 2(4): 423-436. doi: 10.3934/bioeng.2015.4.423

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Abstract

With the emergence of synthetic biology and the vast knowledge about individual biocatalytic reactions, the challenge nowadays is to implement whole natural or synthetic pathways into microorganisms. For this purpose balanced enzyme activities throughout the pathway need to be achieved in addition to simple functional gene expression to avoid bottlenecks and to obtain high titers of the desired product. As the optimization of pathways in a specific biological context is often hard to achieve by rational design, combinatorial approaches have been developed to address this issue. Here, current strategies and proof of concepts for combinatorial pathway assembly in yeasts are reviewed. By exploiting its ability to join multiple DNA fragments in a very efficient and easy manner, the yeast Saccharomyces cerevisiae does not only constitute an attractive host for heterologous pathway expression, but also for assembling pathways by recombination in vivo.

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