Ethanol production from hot-water sugar maple wood extract hydrolyzate: fermentation media optimization for <em>Escherichia coli </em>FBWHR

Yang Wang; Chenhui Liang; Shijie Liu; Yang Wang; Chenhui Liang; Shijie Liu

doi:10.3934/environsci.2015.2.269

AIMS Environmental Science

2015, Volume 2, Issue 2: 269-281. doi: 10.3934/environsci.2015.2.269

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Ethanol production from hot-water sugar maple wood extract hydrolyzate: fermentation media optimization for Escherichia coli FBWHR

Department of Paper and Bioprocess Engineering, State University of New York—College of Environmental Science and Forestry, Syracuse, NY 13210, USA

Received: 25 February 2015 Accepted: 13 April 2015 Published: 19 April 2015

We report the first time statistical study of the optimization for ethanol production from hot-water sugar maple hemicellulosic wood hydrolyzate by Escherichia coli FBWHR. Response surface methodology was employed to investigate the effect of fermentation media on the ethanol production from concentrated hot-water sugar maple hemicellulosic wood extract hydrolyzate by Escherichia coli FBWHR. The critical media components were firstly selected according to Plackett–Burman design and further optimized by central composite design. Based on the response surface analysis, the optimum concentrations of the significant components were obtained: yeast extract, 10.19 g/L; tryptone, 14.55 g/L; Na₂HPO₄·7H₂O, 23.21 g/L; KH₂PO₄, 5 g/L and NH₄Cl, 2 g/L. An ethanol concentration of 15.23 ± 0.21 g/L was achieved under the optimized media, which agreed with the predicted value. Ethanol production was enhanced to 22.18 ± 0.13 g/L by scaling up the fermentation from shaker flask to 1.3 L bioreactor.
- hot-water sugar maple wood hydrolyzate,
- recombinant Escherichia coli,
- ethanol fermentation,
- response surface methodology
Citation: Yang Wang, Chenhui Liang, Shijie Liu. Ethanol production from hot-water sugar maple wood extract hydrolyzate: fermentation media optimization for Escherichia coli FBWHR[J]. AIMS Environmental Science, 2015, 2(2): 269-281. doi: 10.3934/environsci.2015.2.269

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Abstract

We report the first time statistical study of the optimization for ethanol production from hot-water sugar maple hemicellulosic wood hydrolyzate by Escherichia coli FBWHR. Response surface methodology was employed to investigate the effect of fermentation media on the ethanol production from concentrated hot-water sugar maple hemicellulosic wood extract hydrolyzate by Escherichia coli FBWHR. The critical media components were firstly selected according to Plackett–Burman design and further optimized by central composite design. Based on the response surface analysis, the optimum concentrations of the significant components were obtained: yeast extract, 10.19 g/L; tryptone, 14.55 g/L; Na₂HPO₄·7H₂O, 23.21 g/L; KH₂PO₄, 5 g/L and NH₄Cl, 2 g/L. An ethanol concentration of 15.23 ± 0.21 g/L was achieved under the optimized media, which agreed with the predicted value. Ethanol production was enhanced to 22.18 ± 0.13 g/L by scaling up the fermentation from shaker flask to 1.3 L bioreactor.

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