The hybrid nanobiointerface between nitrogen-doped graphene oxide and lipid membranes: a theoretical and experimental study

P. Di Pietro; G. Forte; L. D’Urso; C. Satriano; P. Di Pietro; G. Forte; L. D’Urso; C. Satriano

doi:10.3934/matersci.2017.1.43

AIMS Materials Science

2017, Volume 4, Issue 1: 43-60. doi: 10.3934/matersci.2017.1.43

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The hybrid nanobiointerface between nitrogen-doped graphene oxide and lipid membranes: a theoretical and experimental study

1.
Nanobiohybrid Interfaces Laboratory (NIL), Department of Chemical Sciences, University of Catania, Viale Andrea Doria, 6, I-95125 Catania, Italy
2.
Department of Pharmaceutical Sciences, University of Catania, Viale Andrea Doria, 6, I-95125 Catania, Italy
3.
Department of Chemical Sciences, University of Catania, Viale Andrea Doria, 6, I-95125 Catania, Italy

Received: 31 October 2016 Accepted: 15 December 2016 Published: 21 December 2016

In this study, we present a comparison between graphene oxide (GO) and nitrogen-doped GO (N-GO) in terms of spectroscopic properties and biomolecule-binding potentiality features. Specifically, GO nanosheets, both in aqueous dispersion and in solid state, were successfully modified with different amino-containing moieties, in order to obtain graphene-based nanostructures able to respond to chemical stimuli (e.g., pH) and with tunable surface properties. The physisorption of dye-labelled lipid vesicles loaded with curcumin, was scrutinised both theoretically and experimentally. The energetics of the hybrid lipid membrane-curcumin-GO interface at different pH values, representative respectively of physiological (7.4) and pathological (5.5) environment, were estimated by molecular dynamics (MD) simulations. The GO and GO-N samples characterization by Raman, fluorescence, and UV-vis spectroscopies, as well as confocal microscopy demonstrated promising features of the (N-)GO/lipid platforms for fluorescence imaging and drug delivery applications.
- 2D nanomaterials,
- supported lipid bilayers,
- surface functionalisation,
- molecular dynamics,
- confocal microscopy
Citation: P. Di Pietro, G. Forte, L. D’Urso, C. Satriano. The hybrid nanobiointerface between nitrogen-doped graphene oxide and lipid membranes: a theoretical and experimental study[J]. AIMS Materials Science, 2017, 4(1): 43-60. doi: 10.3934/matersci.2017.1.43

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Abstract

In this study, we present a comparison between graphene oxide (GO) and nitrogen-doped GO (N-GO) in terms of spectroscopic properties and biomolecule-binding potentiality features. Specifically, GO nanosheets, both in aqueous dispersion and in solid state, were successfully modified with different amino-containing moieties, in order to obtain graphene-based nanostructures able to respond to chemical stimuli (e.g., pH) and with tunable surface properties. The physisorption of dye-labelled lipid vesicles loaded with curcumin, was scrutinised both theoretically and experimentally. The energetics of the hybrid lipid membrane-curcumin-GO interface at different pH values, representative respectively of physiological (7.4) and pathological (5.5) environment, were estimated by molecular dynamics (MD) simulations. The GO and GO-N samples characterization by Raman, fluorescence, and UV-vis spectroscopies, as well as confocal microscopy demonstrated promising features of the (N-)GO/lipid platforms for fluorescence imaging and drug delivery applications.

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