Non-invasive analysis of bovine embryo metabolites during <em>in vitro</em> embryo culture using nuclear magnetic resonance

Marcello Rubessa; Andrea Ambrosi; Dianelys Gonzalez-Pena; Kathryn M. Polkoff; Scott E. Denmark; Matthew B. Wheeler; Marcello Rubessa; Andrea Ambrosi; Dianelys Gonzalez-Pena; Kathryn M. Polkoff; Scott E. Denmark; Matthew B. Wheeler

doi:10.3934/bioeng.2016.4.538

AIMS Bioengineering

2016, Volume 3, Issue 4: 538-551. doi: 10.3934/bioeng.2016.4.538

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Research article Special Issues

Non-invasive analysis of bovine embryo metabolites during in vitro embryo culture using nuclear magnetic resonance

1.
Institute for Genomic Biology, University of Illinois, 1207 West Gregory Drive Urbana, IL 62801, USA
2.
Department of Animal Sciences, University of Illinois, 1207 West Gregory Drive Urbana, IL 62801, USA
3.
Department of Chemistry, University of Illinois, 505 S Mathews Ave Urbana, IL 62801, USA

Received: 12 October 2016 Accepted: 02 December 2016 Published: 13 December 2016

The ability to identify embryos that have the highest developmental potential from a cohort would significantly increase the chances of achieving pregnancy. Metabolic analysis is a well-established analytical approach in biological systems. Starting from this idea, we chose to use high-resolution nuclear magnetic resonance (¹H-NMR) spectroscopy. The aim of this study was to determine if it is possible to select viable embryos after 48 h of culture using metabolic activity as the parameter. We evaluated embryo metabolism after the first 48 h of culture and compared the activity of cleaved embryos that became blastocysts to cleaved embryos that did not develop to blastocysts, and in vitro fertilized (IVF) blastocysts and parthenogenetic-activated (PA) blastocysts. Our results show that citrate, pyruvate, myo-inositol and lysine have great impact on predicting embryo development. When we compared IVF and PA blastocysts, we found that acetate and phenylalanine concentrations are excellent parameters for evaluating blastocyst quality. Combining all these results, we were able to create a formula that predicts zygote development after 2 days of culture. In conclusion, we found that it is possible predict the future development of in vitro produced bovine embryos after only 2 days of culture using ¹H-NMR.
- embryo,
- NMR,
- metabolism,
- cattle,
- early development
Citation: Marcello Rubessa, Andrea Ambrosi, Dianelys Gonzalez-Pena, Kathryn M. Polkoff, Scott E. Denmark, Matthew B. Wheeler. Non-invasive analysis of bovine embryo metabolites during in vitro embryo culture using nuclear magnetic resonance[J]. AIMS Bioengineering, 2016, 3(4): 538-551. doi: 10.3934/bioeng.2016.4.538

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Abstract

The ability to identify embryos that have the highest developmental potential from a cohort would significantly increase the chances of achieving pregnancy. Metabolic analysis is a well-established analytical approach in biological systems. Starting from this idea, we chose to use high-resolution nuclear magnetic resonance (¹H-NMR) spectroscopy. The aim of this study was to determine if it is possible to select viable embryos after 48 h of culture using metabolic activity as the parameter. We evaluated embryo metabolism after the first 48 h of culture and compared the activity of cleaved embryos that became blastocysts to cleaved embryos that did not develop to blastocysts, and in vitro fertilized (IVF) blastocysts and parthenogenetic-activated (PA) blastocysts. Our results show that citrate, pyruvate, myo-inositol and lysine have great impact on predicting embryo development. When we compared IVF and PA blastocysts, we found that acetate and phenylalanine concentrations are excellent parameters for evaluating blastocyst quality. Combining all these results, we were able to create a formula that predicts zygote development after 2 days of culture. In conclusion, we found that it is possible predict the future development of in vitro produced bovine embryos after only 2 days of culture using ¹H-NMR.

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